Yonsei Med J.  2008 Dec;49(6):949-954. 10.3349/ymj.2008.49.6.949.

DNA Gains and Losses of Chromosome in Laryngeal Squamous Cell Carcinoma Using Comparative Genomic Hybridization

• Keser I
• Toraman AD
• Ozbilim G
• Guney K
• Luleci G

• KMID: 1782943
• DOI: http://doi.org/10.3349/ymj.2008.49.6.949

Abstract

PURPOSE
A larynx squamous cell carcinoma (LSCC) is one of the most common forms of cancer and may exhibit various complex karyotypes. MATERIALS AND METHODS: We used comparative genomic hybridization (CGH) to analyze DNA gains and losses in 15 squamous cell carcinomas that consisted of 4 glottic, 10 supraglottic, and 1 transglottic localization samples. RESULTS: The majority of the chromosomal alterations detected were gains: 3 samples of LSCCs revealed high level amplification, while 6 samples displayed gains in various chromosomal regions (17p, 3p, 4p, 5p, 6q, 8p, 9p, 14q, 18p and Xq). One sample was found to have losses (chromosomes 15q and 22q) and 5 had normal CGH profiles. CONCLUSION: Many of these gained regions (4p, 5p, 8p, 10q, 18q and Xq) were novel sites, which may harbor oncogene(s) that potentially play an important role in squamous cell tumorigenesis and progression at supraglottic localizations.

Keyword

Larynx cancer; squamous cell carcinoma; comparative genomic hybridization; deoxyribonucleic acid

MeSH Terms

Adult
Aged
Carcinoma, Squamous Cell/*genetics
*Chromosome Aberrations
Comparative Genomic Hybridization
DNA, Neoplasm/*genetics/*metabolism
Female
Humans
Karyotyping
Laryngeal Neoplasms/*genetics
Male
Middle Aged

Figure

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  Fig. 1 Summary of chromosomal gains and losses detected by comparative genomic hybridization in fifteen larynx carcinomas. The Y chromosome, acrocentric p-arms, and chromosome centromeric heterochromatin regions were excluded from the analysis. Vertical lines on the right and left sides of a chromosome indicate a gain or loss of genetic material, respectively.

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