Korean J Gastroenterol.  2002 Apr;39(4):275-283.

Genetic Alterations in Colorectal Carcinoma Detected by Comparative Genomic Hybridization

• Park SJ
• Park MI
• Koo SH
• Koo JY

Abstract

BACKGROUND/AIMS: Comparative genomic hybridization (CGH) is a new powerful technique for the molecular cytogenetic analysis of cancer.
METHODS
Twenty-five colorectal carcinomas were screened for chromosomal aberrations using CGH.
RESULTS
All carcinomas had chromosomal aberrations, and the mean number of chromosomal aberrations per tumor was 5.3. The cases of chromosomal gains were more common than the cases of chromosomal losses. The cases of amplification of 7q region, which contains locus of c-met, was significantly higher in stage III and IV than in stage I and II (p<0.05). In three cases of stage IV (67%), two showed amplification of 7q and 20q, in which c-met oncogene and c-src oncogene are located, respectively. The chromosomal gains of tumor were mostly detected at 20q, 7q, and 13q. The chromosomal losses of tumor were frequently detected at 17p and 18q.
CONCLUSIONS
The CGH may be useful in predicting the prognosis or therapeutic decision of colorectal carcinoma. Especially, the chromosomal changes at 7q, 20q and 13q as well as c-met and c-src may be useful markers for colorectal carcinoma.

Keyword

Colorectal cancer; Gene; Gain; Loss; Comparative genomic hybridization

MeSH Terms

Chromosome Aberrations
Colorectal Neoplasms*
Comparative Genomic Hybridization*
Cytogenetic Analysis
Oncogenes
Prognosis