Korean J Hepatobiliary Pancreat Surg.  1999 Aug;3(2):77-88.

A study on the chromosomal aberrations in Korean intrahepatic cholangiocarcinomas with comparative genomic hybridization

• Choi SH
• Sohn TS
• Joh JW
• Kim SJ
• Heo JS
• Kim YI
• Lee KS
• Cho YH

Abstract

AIMS AND METHOD: Comparative genomic hybridization serves as a screening test for regions of copy number changes in tumor genomes. I have applied the technique to map DNA losses and gains in 13 cases of frozen cholangiocarcinomas.
RESULTS
All of the 13 cases showed genetic alterations. Loss of short arm of chromosome 19 (92%) was the most common changes observed. 22q(62%), 1p(54%), 17p(54%) and 19q(54%) also showed nonrandom patterns of genomic losses with high frequencies. Among the genomic gains, 13q was revealed as the most common site (69%), and 8q (46%) and 12q (46%) also showed relatively high frequencies of genomic gains. Genomic amplifications were detected on 5p13, 10q21.1 and 18q11.3 in 3 different cases, respectively.
CONCLUSION
This study represents the first analysis of intrahepatic cholangiocarcinomas by CGH, and it confirms the presence of nonrandom genetic changes occur in the pathogenesis of cholangiocarcinomas. These findings should lead to the characterization of new loci involved in cholangiocarcinoma pathogenesis.

Keyword

Comparative genomic hybridization; Intrahepatic cholangiocarcnoma

MeSH Terms

Arm
Cholangiocarcinoma*
Chromosome Aberrations*
Chromosomes, Human, Pair 19
Comparative Genomic Hybridization*
DNA
Genome
Mass Screening
DNA