Korean J Fertil Steril.  1998 Apr;25(1):87-92.

In Vitro/In Vivo Development of Mouse Oocytes Vitrified by EFS

• Kim MK
• Kim EY
• Yi BK
• Yoon SH
• Park SP
• Chung KS
• Lim JH

Abstract

SUMMARY This study was carried out to investigate in vitro/in vivo development of vitrified mouse oocytes. Mouse oocytes were vitrified using EFS30, 35 and 40 (30, 35 and 40% ethylene glycol, 18% ficoll and 0.5 M sucrose in M2medium). After being exposed or vitrified-thawed, oocytes of normal morphology were inseminated in vitro by 1-2x 106/m1 of epididymal sperm. The rates of fertilization, in vitro/in vivo development and cell number(inner cell mass and tropectoderm cell) of blastocysts in each treatment group were examined. The results obtained in these experiments were summarized as follows: The cleavage rates were obtained in EFS35 containing 35% ethylene glycol higher than in EFS30 and EFS40. The development rate of vitrified-thawed oocytes to two-cell stage after in vitro fertilization (S 1.1%) was significantly different compared to that of exposed to vitrification solution without cooling (60.0%) and control (68.2%) (p<0.05). However, there were no differences in the blastocyst formation from the cleaved embryos among groups (75.0, 73.3 and 80.0%). Also, the clean number of cells per blastocysts of vitrified group (92.5 +/- 2.9) was similar to that of the exposed (98.5 +/- 5.3) and control (100.9 +/- 4.8). In vivo development of the blastocysts derived from vitrified-thawed oocytes resulted in fetal development (50.7%) and implantation rates (80.0%) which are very similar to those of control (58.2, 78.2%). These results suggest that mouse oocytes could be cryopreserved using vitrification solution (EFS35) based on ethylene glycol.

MeSH Terms

Animals
Blastocyst
Embryonic Structures
Ethylene Glycol
Fertilization
Fertilization in Vitro
Fetal Development
Ficoll
Mice*
Oocytes*
Spermatozoa
Sucrose
Vitrification
Ethylene Glycol
Ficoll
Sucrose