The Oral Therapy of Flavonoids Derivative DA-6034 in the Experimental Animal Models of Inflammatory Bowel Disease

Kim, You Sun; Kim, Tae Ho; Jung, Hyun Chae; Song, In Sung; Kim, Chung Yong; Ko, Jun Il; Son, Mi Won; Kim, Won Bae

Abstract

BACKGROUND/AIMS: Eupatilin, a kind of flavonoids, has been known to be effective for chronic diarrhea in Korea. We performed this study to evaluate the oral therapeutic effect of DA-6034, a synthetic derivative of Eupatilin, in two experimental animal models.
METHODS
The Sprague- Dawley rats were used. Trinitrobenzene sulfonic acid (TNBS)-enteritis was induced by injection of TNBS solution at distal ileum after laparotomy. Then, the subjects were categorized into four groups. Each group was administered orally saline, 3 mg/kg of DA-6034, 1 mg/kg of prednisolon (Pd) or 100 mg/kg of sulfasalazine, respectively, for 2 weeks. HLA-B27 transgenic rats, in which spontaneous colitis is known to develop, were categorized into three groups and each group was administered orally saline, 3 mg/kg of DA-6034 and 0.5 mg/kg of Pd for 6 weeks. The therapeutic effects were assessed by gross and microscopic damage score and the level of leukotriene B4 (LTB4) and prostaglandin E2 (PGE2).
RESULTS
In TNBS-enteritis induced rats, the gross damage score and the level of LTB4 of the groups treated with DA-6034, Pd, and sulfasalazine were significantly lower than those of saline group (p<0.05). However, there was no difference among those of the treatment groups. In HLA-B27 transgenic rats, the microscopic damage score of treatment groups was significantly decreased compared with saline group (p<0.05) and the level of PGE2 showed decreasing tendency.
CONCLUSIONS
DA-6034 has significant therapeutic effects on two animal models with inflammatory bowel disease. Therefore, it is worth to have human trial for the patients with inflammatory bowel disease.