Korean J Nephrol.  2006 Jan;25(1):13-22.

Expression of Platelet Derived Growth Factor-A, C and Platelet Derived Growth Factor Receptor-alpha in the Ischemia Reperfusion Renal Failure Model

• Kang KP
• Kim W
• Moon CY
• Jang YB
• Lee S
• Moon SO
• Sung MJ
• kIm DH
• Kang SK
• Park SK

Abstract

BACKGOUND: Platelet-derived growth factor (PDGF) is a widely expressed growth factor with both mitogenic and chemotactic activities in many connective tissue cell types. There are four members of PDGF family; PDGF-A, PDGF-B, PDGF-C, PDGF-D. Their biological effects are mediated via two tyrosine kinase receptors, PDGF receptor-alpha and PDGF receptor-beta, and PDGF-mediated signaling is critical for development of many organ systems and acquired disease. The aims of this study were to determine the changes of PDGF-A, PDGF-C and PDGF receptor (PDGFR)-alpha expression in ischemia reperfusion acute renal failure model.
METHODS
We examined the expression and localization of PDGF-A, PDGF-C and PDGF receptor-alpha protein using Western blot analysis and immunohistochemistry and PDGF-C mRNA using RNase protection assay after ischemia reperfusion renal failure model.
RESULTS
PDGF-A expression showed no change after ischemia reperfusion injury. Proliferating cell nuclear antigen expression increased at day 2 after ischemia reperfusion injury. PDGF-C expression increased at day 2 after ischemia reperfusion injury, and was localized in tubular epithelial cells of outer medulla. PDGFR-alpha increased at day 2 after ischemia reperfusion injury, and was localized in tubular interstitium of outer medulla.
CONCLUSION
These results indicated that PDGF-C and PDGF receptor-alpha may have an important role in the renal regeneration after ischemia reperfusion renal injury.

Keyword

Platelet-derived growth factor; Ischemia/reperfusion injury; Kidney failure; Acute

MeSH Terms

Acute Kidney Injury
Blood Platelets*
Blotting, Western
Connective Tissue Cells
Epithelial Cells
Humans
Immunohistochemistry
Ischemia*
Platelet-Derived Growth Factor*
Proliferating Cell Nuclear Antigen
Receptor Protein-Tyrosine Kinases
Receptors, Platelet-Derived Growth Factor
Regeneration
Renal Insufficiency*
Reperfusion Injury
Reperfusion*
Ribonucleases
RNA, Messenger
Platelet-Derived Growth Factor
Proliferating Cell Nuclear Antigen
RNA, Messenger
Receptor Protein-Tyrosine Kinases
Receptors, Platelet-Derived Growth Factor
Ribonucleases