Korean J Obstet Gynecol.  2001 Sep;44(9):1633-1638.

Expression analysis of genes related to multidrug resistance (MDR) in ovarian cancer cell line A2780 and cisplatinum resistant cell line A2780cp

• Lee JK
• Na YJ
• Lee YM
• Chang SY
• Moon YJ
• Cho SH
• Kim KT
• Hwang YY

Abstract

OBJECTIVE
Expressions of P-glycoprotein, the multidrug resistance-associated protein (MRP) and the lung resistance protein (LRP) with the MDR phenotype widely divergent in human cancer cell lines. This study focused on the altered gene expression related drug transport.
METHODS
To examine correlations between MDR-associated genes and PKC isozyme with cisplatin resistance on the level of the mRNA expression, we analyzed MDR-associated gene (LRP, MDR1/P-gp and MRP) expression and PKC isozyme, topoisomerase II alpha and beta in cisplatin-sensitive ovarian cancer cell line A2780 and cisplatin - resistant cell line A2780cp using cDNA-PCR approach.
RESULTS
LRP mRNA levels were significantly increased in A2780cp compared to the drug sensitive variant. In contrast, MRP mRNA levels were not significantly correlated with drug sensitivity. A modest increase in PKC(eta) and MDR1/P-gp mRNA expression activity was also observed in ovarian cancer A2780cp cell lines that were resistant to CDDP. The level of topoisomerase II alpha and beta were not affected.
CONCLUSION
These results showed that MDR1/P-gp expression may be an important determinant of the MDR phenotype in cell lines intrinsically resistant to cancer chemotherapeutic agents and a multifactorial emergence of MDR phenotype of tumor with a possible involvement of the PKC isozymes may be associated with CDDP resistant ovarian cancer cell line.

Keyword

Genes; MDR; Ovarian neoplasm; Reverse transcriptase polymerase chain reaction

MeSH Terms

Cell Line*
Cisplatin
DNA Topoisomerases, Type II
Drug Resistance, Multiple*
Gene Expression
Humans
Isoenzymes
Lung
Multidrug Resistance-Associated Proteins
Ovarian Neoplasms*
P-Glycoprotein
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Cisplatin
DNA Topoisomerases, Type II
Isoenzymes
Multidrug Resistance-Associated Proteins
P-Glycoprotein
RNA, Messenger