Change of Chemosensitivity and Cellular Characteristics of Cisplatinum Resistant Human Bladder Cancer Cells Induced by Long-term Cisplatinum Treatment

Lee, Eunsik; Min, Kyung Joon; Kim, Soo Woong; Lee, Hae Won; Cho, Kyu Seo; Kim, Kwang Myung; Lee, Sang Eun; Lee, Chongwook

Abstract

PURPOSE: Cisplatinum has the therapeutic efficacy against a bladder cancer. However, the response is often limited due to appearance of drug-resistant tumor cells. The studies of establishment and characterization of cisplatinum-resistant tumor cells are considered to be helpful in elucidating the underlying mechanism of acquired resistance to cisplatinum in human tumors.
MATERIALS AND METHODS
We established cisplatinum-resistant cell lines sequentially, T24Rl and T24R2, which show resistance to cisplatinum at a concentration of 1 and 2microgram /ml, respectively, by the stepwise exposure of T24 human bladder cancer cell to increasing concentrations of cisplatinum.
RESULTS
The resistance to cisplatinum of T24Rl and T24R2 cells was 13- and 18-fold that of the parental T24 cells, respective1y. Growth, DNA synthesis and cell cycle distribution of T24Rl and T24R2 cells were not different from those of the parental T24 cells.
CONCLUSIONS
These cisplatinum-resistant bladder tumor cell lines could be a useful model to elucidate the biochemical and molecular mechanism Involved In the cisplatinum resistance.