The significance of glucose consumption in evaluation of nephrotoxicity on histocultured human renal cortical tissue

Kim, Ki Kyung; Chai, Soo Eung

Abstract

The ability to support the heterogenous cell population having in vivo growth characteristics for in vitro study has been the goal of many investigators. In 1986, an assay offering that three dimensional culture system grows tumors obtained directly from surgery or biopsy at high frequency for long periods of time and has them maintain many of their in vivo properties was reported. In this study, these experimental model for three-dimensional ""native-state"" culture of tissues or collagen gels have been applied to normal human renal cortical tissues obtained directly from the nephrectomized kidney for renal cell carcinoma. Gel specimens histocultured for 4 weeks were treated with cisplalinum with a variety of concentrations and exposure times. Pre-treatment viability of the gel specimens was checked by measuring the amount of glucoseconsumption by viable tissues. Cisplatinum induced toxicities were evaluated by measuring of glucose consumption and [3H] thymidine incorporation. The glucose consumption of renal cortical tissues decreased steadily in the control group. Cisplatinum induced suppression of glucose consumption, starting on 2x 10-5M in concentration, was proportionally increased by concentration and duration of exposure. These results may provide an experimental availability for the three dimensional collagen gel culture method applied to human renal cortical tissue as a tissue culture model and for the glucose consumption as an index representing tissue viability as a whole. It was not found that glucose consumption rate was related to thymidine uptake as a DNA precursor incorporation.