Gut Liver.  2014 Nov;8(6):632-636. 10.5009/gnl13224.

Investigation of -308G>A and -1031T>C Polymorphisms in the TNFA Promoter Region in Polish Peptic Ulcer Patients

• Salagacka
• Zebrowska
• Jelen A
• Mirowski M
• Balcerczak E

• KMID: 2070080
• DOI: http://doi.org/10.5009/gnl13224

Abstract

BACKGROUND/AIMS
Tumor necrosis factor alpha (TNF-alpha) encoded by TNFA is a key mediator in inflammation, a precursor condition for peptic ulceration. Promoter polymorphisms of TNFA that influence its transcriptional activity and TNF-alpha production are known. TNFA-308G>A (rs1800629) and TNFA-1031T>C (rs1799964), which are responsible for increased TNFA transcription, could influence the risk of peptic ulceration. This study aimed to investigate these polymorphisms and to evaluate their association with peptic ulcer disease and Helicobacter pylori infection in the Polish population.
METHODS
Gastric mucosa specimens obtained from 177 Polish peptic ulcer patients were used to conduct rapid urease tests and to assess the investigated polymorphisms by polymerase chain reaction-restriction fragment length polymorphism. Genotyping data were compared with the results obtained from healthy individuals of Polish origin.
RESULTS
There were no significant differences in genotype and allele frequency of the investigated polymorphisms between peptic ulcer patients and healthy individuals. No associations between the frequencies of particular genotypes and alleles for both single-nucleotide polymorphisms (SNPs) and the presence of H. pylori infection in peptic ulcer patients and in subgroups of men and women with peptic ulcer disease were found.
CONCLUSIONS
The investigated SNPs are not risk factors for either peptic ulcer or H. pylori infection development in the Polish population. The results require verification in a larger cohort.

Keyword

Tumor necrosis factor-alpha; Genetic polymorphism; Peptic ulcer; Restriction fragment length polymorphism

MeSH Terms

Adolescent
Adult
Aged
Aged, 80 and over
Case-Control Studies
European Continental Ancestry Group/genetics
Female
Gastric Mucosa/*metabolism/microbiology
Genetic Predisposition to Disease
Genotype
Helicobacter Infections/complications/*genetics
Helicobacter pylori
Humans
Male
Middle Aged
Peptic Ulcer/complications/*genetics
Poland
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
*Promoter Regions, Genetic
Tumor Necrosis Factor-alpha/*genetics
Young Adult
Tumor Necrosis Factor-alpha