Int J Oral Biol.  2015 Sep;40(3):111-116. 10.11620/IJOB.2015.40.3.111.

Root Resorption in Streptozotocin-induced Diabetic Rats with Ligature-induced Periodontitis

• Kim JH
• Lee DE
• Park JC
• Kim YJ
• Cha JH
• Bak EJ
• Yoo YJ

• KMID: 2068540
• DOI: http://doi.org/10.11620/IJOB.2015.40.3.111

Abstract

To determine the effect of diabetes on root resorption in periodontitis, we investigated odontoclast formation and root resorption in diabetic rats with periodontitis. Odontoclast formation was observed in three groups of F344 rats: Controls (C) were normal rats without diabetes or periodontitis; the periodontitis (P) group had mandibular first molars to be ligatured; the periodontitis with diabetes (PD) group was intravenously administered streptozotocin (50 mg/kg) to induce diabetes and had mandibular first molars to be ligatured. On days 3, 10, and 20 after ligature, tumor necrosis factor (TNF)-alpha and receptor activator of nuclear factor-kappaB ligand (RANKL) expression, odontoclast formation, and root resorption areas were evaluated by immunohistochemistry, tartrate-resistant acid phosphatase staining, and hematoxylin and eosin staining, respectively. The PD group showed frequent urination, weight loss, and hyperglycemia. Numbers of TNF-alpha- and RANKL-positive cells were higher in the P and PD groups than in the C group. It was more prevalent in PD group on day 3. Odontoclast formation was greater in the P and PD groups than in the C group on days 3 and 10, then decreased to same level as the C group by day 20. Root resorption in the PD and P groups showed increases on days 3 and 10, respectively, compared to the C group. These results suggest that diabetes may transiently increase root resorption on day 3 with high expression of TNF-alpha and RANKL after periodontitis induction. This study could aid the understanding of root resorption in diabetic patients with periodontitis.

Keyword

Diabetes Mellitus; Odontoclasts; Periodontitis; Root Resorption

MeSH Terms

Acid Phosphatase
Animals
Diabetes Mellitus
Eosine Yellowish-(YS)
Hematoxylin
Humans
Hyperglycemia
Immunohistochemistry
Ligation
Molar
Osteoclasts
Periodontitis*
RANK Ligand
Rats*
Rats, Inbred F344
Root Resorption*
Streptozocin
Tumor Necrosis Factor-alpha
Urination
Weight Loss
Acid Phosphatase
Eosine Yellowish-(YS)
Hematoxylin
RANK Ligand
Streptozocin
Tumor Necrosis Factor-alpha