J Korean Geriatr Soc.  1997 Oct;1(1):48-54.

Proliferation of Cultured Vascular Smooth Muscle Cells(VSMCs) Obtained from Aortas of Insulin Dependent Diabetic Rats

• Yoo HJ

Abstract

BACKGROUND: Vascular smooth muscle cells(VS-MCs) from diabetic animals proliferate more than that from non-diabetic ones. The proliferative ability of cultured VSMCs is changed by the kind of experimental animals, experimental technique and so on. In this study, I used VSMCs from streptozotocin-induced insulin dependent diabetic Sprague Dawley rats and examined the characteristics of aortic VSMCs proliferation from these rats.
METHODS
Using enzyme method I cultured aortic VSMCs. I compared the proliferation between normal rat VSMCS and diabetic ones. I studied the cell growth of VSMCs from diabetic rats as a function of period after streptozotocin injection. I also examined the effects of hyperglycemia and insulin treatment on the proliferation of cultured VSMCs.
RESULTS
Diabetic rats VSMCs proliferated more than non-diabetic ones in streptozotocin-induced insulin dependent diabetic rats. The proliferation of VSMCs was enhanced from 2 weeks after streptozotocin injection. Hyperglycemia enhanced the proliferation and insulin treatment lowered that.
CONCLUSIONS
The proliferation of VSMCs was increased in streptozotocin-induced diabetic rats.

Keyword

Diabetic rat; Streptozotocin; Vascular smooth muscle cell; Proliferati

MeSH Terms

Animals
Aorta*
Hyperglycemia
Insulin*
Muscle, Smooth, Vascular*
Rats*
Rats, Sprague-Dawley
Streptozocin
Insulin
Streptozocin