Korean J Otorhinolaryngol-Head Neck Surg.  2015 Sep;58(9):615-621. 10.3342/kjorl-hns.2015.58.9.615.

Effect of Polyinosinic-Polycytidylic Acid on MUC5B Expression in Human Airway Epithelial Cells

Affiliations
  • 1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr
  • 2Regional Center for Respiratory Diseases, Yeungnam University Medical Center, Daegu, Korea.

Abstract

BACKGROUND AND OBJECTIVES
Polyinosinic-polycytidylic acid (Poly I:C) is structurally similar to double-stranded RNA, and is known to induce various inflammatory mediators and to cause inflammatory reactions in airway epithelial cells. However, the effect of Poly I:C on secretion of mucins in human airway epithelial cells has been very rarely reported. In this study, the effect and brief signaling pathway of Poly I:C on the expression of mucin genes were investigated in human airway epithelial cells. MATERIALS AND METHOD: In mucin-producing human NCI-H292 airway epithelial cells and the primary cultures of normal human nasal epithelial cells, the effect and signaling pathway of Poly I:C on expression of mucin genes were investigated using reverse transcriptase-polymerase chain reaction, real-time PCR, enzyme immunoassay, and immunoblot analysis with specific inhibitors and small interfering RNA (siRNA) for mitogen-activated protein kinase (MAPK).
RESULTS
Poly I:C induced the MUC5B expression, and activated the phosphorylation of ERK1/2 and p38 MAPK. U0126 (ERK1/2 MAPK inhibitor) and SB203580 (p38 MAPK inhibitor) inhibited the Poly I:C-induced MUC5B expression. In addition, the knockdown of ERK2 and p38 MAPK by siRNA significantly blocked the Poly I:C-induced MUC5B mRNA expression.
CONCLUSION
Poly I:C induces the MUC5B expression via ERK2 and p38 MAPK signaling pathways in human airway epithelial cells. Therefore, Poly I:C may play a role in the regulation of mucus hypersecretion through MAPK signaling pathways in the human airway epithelial cells.

Keyword

Human airway epithelial cells; Mitogen-activated protein kinase; MUC5B; Polyinosinic-polycytidylic acid

MeSH Terms

Epithelial Cells*
Humans*
Immunoenzyme Techniques
Mucins
Mucus
p38 Mitogen-Activated Protein Kinases
Phosphorylation
Poly I-C*
Protein Kinases
Real-Time Polymerase Chain Reaction
RNA, Double-Stranded
RNA, Messenger
RNA, Small Interfering
Mucins
Poly I-C
Protein Kinases
RNA, Double-Stranded
RNA, Messenger
RNA, Small Interfering
p38 Mitogen-Activated Protein Kinases
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