J Korean Breast Cancer Soc.  2002 Dec;5(4):291-297. 10.4048/jkbcs.2002.5.4.291.

Significance of the Expression of p27(Kip1) Protein in Human Breast Cancer

• Kim CY
• Yang KH
• Bae BN
• Kim KH
• Kim HJ
• Kim YD
• Kim HY
• Park K
• Han S

• KMID: 2059063
• DOI: http://doi.org/10.4048/jkbcs.2002.5.4.291

Abstract

PURPOSE: p27(Kip1) is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. It binds to a variety of cyclin/CDK complexes, inhibits kinase activity and blocks the cell cycle as a negative regulator. Reduced p27(Kip1) expression has been reported to be a significant predictor of poor survival in numerous human breast cancers. We executed p27(Kip1) protein assay in primary breast cancer and compared these result with known prognostic parameters.
METHODS
Immunohistochemical assay was performed on tissue microarrays from 183 patients with breast cancer to evaluate the biologic and clinical significance of p27(Kip1) expression.
RESULTS
Decreased p27(Kip1) expression was significantly associated with clinical stage (P=0.027), low nuclear grade (P<0.001), high histologic grade (P<0.001), high score mitotic index (P<0.001), increased Ki67 labeled index (P=0.006) and negative estrogen receptor status (P=0.0024). In survival analysis, p27(Kip1) was useful to predict disease- free survival (P=0.0106) and overall survival (P=0.0154) of the patient after surgery followed by chemotherapy or radiation therapy.
CONCLUSION
Reduced expression of p27(Kip1) protein was as sociated with biologically aggressive phenotype of breast cancer and was an useful to predict patient outcome.

Keyword

Breast cancer; p27(Kip1); Tissue microarrays; Prognosis

MeSH Terms

Breast Neoplasms*
Breast*
Cell Cycle
Drug Therapy
Estrogens
Humans*
Mitotic Index
Phenotype
Phosphotransferases
Prognosis
Estrogens
Phosphotransferases