J Korean Breast Cancer Soc.  2004 Jun;7(2):98-103. 10.4048/jkbcs.2004.7.2.98.

Different Expression of p27(kip1) and p57(kip2) by Genistein Treatment in MDA-MB-231 Human Breast Cancer Cells

• Yoon DS
• Song KH
• Kim ST

• KMID: 1917896
• DOI: http://doi.org/10.4048/jkbcs.2004.7.2.98

Abstract

PURPOSE
The isoflavones in soy are likely to contribute to the historically low incidence of breast cancer among Asian women who consume traditional diets. The possible role of isoflavones in controlling the expression of p27(kip1) and p57(kip2) has not been previously explored. In this study, the ability of the isoflavone, genistein, to regulate the expression of p27(kip1) and p57(kip2) in breast cancer cells was evaluated. METHODS: The effects of genistein was examined on the expression of p27(kip1) and p57(kip2) at the mRNA level, using the MDA-MB 231 human breast cancer cell lines. RESULTS: By genistein treatment, p27(kip1) mRNA increased significantly in comparison to the control group. In particular, p27(kip1) mRNA level in 10 micrometer and 50 micrometer genistein treated- groups increased about two-folds more than the control. p57(kip2) mRNA was not different from control group. p57(kip2) mRNA decreased slightly only in the 50 micrometer genistein treated-group. PPAR-gamma mRNA level increased slightly according to the concentration-dependent manner of genistein, but it was not significantly different.
Conclusions
These results suggest that the up-regulated p27(kip1) by genistein might contribute to cancer prevention or inhibition.

Keyword

Breast cancer; p27(kip1); p57(kip2); Genistein

MeSH Terms

Asian Continental Ancestry Group
Breast Neoplasms*
Breast*
Cell Line
Diet
Female
Genistein*
Humans*
Incidence
Isoflavones
RNA, Messenger
Genistein
Isoflavones
RNA, Messenger