Korean J Obstet Gynecol.  2004 Mar;47(3):545-550.

The Expression of p27(kip1) and p57(kip2) in Mouse Endometrium

• Kim ST
• Lee SK
• Gye MC

Abstract

OBJECTIVE
This study was to investigate the expression of CDK inhibitors, p27(kip1) and p57(kip2) in mouse endometrium during the estrus cycle and pregnant period.
METHODS
Total RNA and protein were extracted from endometrium of mouse sacrificed at diestrus, proestrus, estrus, and metestrus cycle, and at day 1-6 post-coitum (p.c.), then semi-quantitative RT-PCR and western blotting of p27(kip1) and p57(kip2) was carried out.
RESULTS
p27(kip1) and p57(kip2) mRNA was highly expressed in diestrus and proestrus stage than estrus and metestrus stage. In comparison with estrus cycle, p27(kip1) and p57(kip2) mRNA level was highly maintained in gestational endometrium (except p27(kip1) of day 5 p.c). p57(kip2) protein level was relatively low from day 1 p.c. to day 4 p.c. But it was significantly increased in day 5 p.c. and day 6 p.c.
CONCLUSION
These results show that p27(kip1) and p57(kip2) may play a role in endometrial differentiation for regular estrus cycle and implantation, and especially p57(kip2) may play an essential role in endometrial differentiation for maintenance of implantation.

Keyword

Mouse endometrium; p27(kip1); p57(kip2)

MeSH Terms

Animals
Blotting, Western
Diestrus
Endometrium*
Estrus
Female
Metestrus
Mice*
Proestrus
RNA
RNA, Messenger
RNA
RNA, Messenger