J Korean Acad Oral Maxillofac Radiol.  1997 Feb;27(1):141-166.

Ultastructural study of the fibroblast remodeling in the radiation: impaired wound healing in the tongue of the rat

• Lee JK
• Choi SC
• Park TW
• You DS

Abstract

Radiation-imparied wound healing in animal experiments was believed to be an another logical experimental model to understand the wound healing mechanism in patients. the purpose of this study was to reveal the block point which would result impaired healing. Twenty four rats(Sprague-Dawley stains) were divided into two gruops according to the time interval between irradiati on and wounding. Group I, observing the healing effect on the 1st day and group II are the healing effects on the 7th d ays after irradiation to the wound of the rat tongue. Experimental animals were sacrificed 3, 6, 12 and 24 hours after w ounding. The specimens were examined by the light microscope and trasmission electron microscope. The following results were obtained: 1. Fibroblasts in both goups showed degenerative changes which were dilated mitochondria and rER, reduced microorganell e, vacuoles and little cytoplasmic process. 2. Average length between bands and quantity of the newly produced collagen fibers aroud fibroblasts remained unchanged against control group. 3. The severity of degenerative change of the fibroblast and impairment of wond healing including shortening of the thi ckness of collagen fibers were more sever in the group II than in the group I.

MeSH Terms

Animal Experimentation
Animals
Collagen
Cytoplasm
Fibroblasts*
Humans
Logic
Mitochondria
Models, Theoretical
Rats*
Tongue*
Vacuoles
Wound Healing*
Wounds and Injuries*
Collagen