Immune Netw.  2008 Mar;8(1):29-37. 10.4110/in.2008.8.1.29.

IL-23 P19 Expression Induced by IL-17 and IL-1beta in Rheumatoid Arthritis Synovial Mononuclear Cells

• Cho ML
• Heo YJ
• Oh HJ
• Kang CM
• Lee SY
• Hong YS
• Kim HY

• KMID: 2054256
• DOI: http://doi.org/10.4110/in.2008.8.1.29

Abstract

Interleukin-23 (IL-23) is a novel pro-inflammatory cytokine which has been implicated to play a pathogenic role in rheumatoid arthritis (RA). This study was undertaken to investigate the IL-23 inductive activity of the proinflammatory cytokine IL-17, IL-1 beta and tumor necrosis factor (TNF-alpha) in RA synovial fluid mononuclear cells (SFMC). Expression of IL-23p19, IL-17, IL-1 beta and TNF-alpha in joint was examined by immunohistochemistry (IHC) of patients with RA and osteoarthritis (OA). The effects of IL-17 and IL-1 beta on expression of IL-23p19 in human SFMC from RA patients were determined by reverse transcriptase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). IL-23p19 was expressed in the RA fibroblast like synoviocyte (FLS), but not from OA FLS. Similar to the protein expression, IL-23p19 mRNA could be detected by RT-PCR in RA SFMC. IL-17 and IL-1 beta could induce RA SFMC to produce the IL-23p19. The effects of IL-17 were much stronger than IL-1 beta or TNF-alpha. These responses were observed in a dose- responsive manner. In addition, IL-17 or IL-1 beta neutralizing antibody down-regulated the expression of IL-23p19 induced by LPS in RA-SFMC. Our results demonstrate that IL-23p19 is overexpressed in RA synovium and IL-17 and IL-1 beta appears to upregulate the expression of IL-23p19 in RA-SFMC.

Keyword

Rheumatoid arthritis; Synovial fluid mononuclear cells; IL-23p19; IL-17; IL-1 beta

MeSH Terms

Antibodies, Neutralizing
Arthritis, Rheumatoid
Enzyme-Linked Immunosorbent Assay
Fibroblasts
Humans
Immunohistochemistry
Interleukin-17
Interleukin-1beta
Interleukin-23
Interleukin-23 Subunit p19
Joints
Osteoarthritis
RNA, Messenger
RNA-Directed DNA Polymerase
Synovial Fluid
Synovial Membrane
Tumor Necrosis Factor-alpha
Antibodies, Neutralizing
Interleukin-17
Interleukin-1beta
Interleukin-23
Interleukin-23 Subunit p19
RNA, Messenger
RNA-Directed DNA Polymerase
Tumor Necrosis Factor-alpha