Exp Neurobiol.
2011 Jun;20(2):100-109. 10.5607/en.2011.20.2.100.
Protective Effect of Coriolus versicolor Cultivated in Citrus Extract Against Nitric Oxide-Induced Apoptosis in Human Neuroblastoma SK-N-MC Cells
- Affiliations
-
- 1Department of Anatomy-Pointology, College of Oriental Medicine, Kyungwon University, Seongnam, Korea.
- 2Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea. changju@khu.ac.kr
- 3Acupuncture and Meridian Science Research Center, Kyung Hee University, Seoul, Korea.
- 4Division of Biotechnology and Collaborative Institute of Science and Technology, The Catholic University of Korea, Bucheon, Korea.
- KMID: 2052822
- DOI: http://doi.org/10.5607/en.2011.20.2.100
Abstract
- Nitric oxide (NO) is a reactive free radical and a messenger molecule in many physiological functions. However, excessive NO is believed to be a mediator of neurotoxicity. The medicinal plant Coriolus versicolor is known to possess anti-tumor and immune-potentiating activities. In this study, we investigated whether Coriolus versicolor possesses a protective effect against NO donor sodium nitroprusside (SNP)-induced apoptosis in the human neuroblastoma cell line SK-N-MC. We utilized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme activity assay in SK-N-MC cells. MTT assay showed that SNP treatment significantly reduces the viability of cells, and the viabilities of cells pre-treated with the aqueous extract of Coriolus versicolor cultivated in citrus extract (CVEcitrus) was increased. However, aqueous extract of Coriolus versicolor cultivated in synthetic medium (CVEsynthetic) showed no protective effect and aqueous citrus extract (CE) had a little protective effect. The cell treated with SNP exhibited several apoptotic features, while those pre-treated for 1 h with CVEcitrus prior to SNP expose showed reduced apoptotic features. The cells pre-treated for 1 h with CVEcitrus prior to SNP expose inhibited p53 and Bax expressions and caspase-3 enzyme activity up-regulated by SNP. We showed that CVEcitrus exerts a protective effect against SNP-induced apoptosis in SK-N-MC cells. Our study suggests that CVEcitrus has therapeutic value in the treatment of a variety of NO-induced brain diseases.
MeSH Terms
-
Apoptosis
Blotting, Western
Brain Diseases
Caspase 3
Cell Line
Citrus
DNA Fragmentation
DNA Nucleotidylexotransferase
Flow Cytometry
Humans
Indoles
Neuroblastoma
Nitric Oxide
Nitroprusside
Plants, Medicinal
Tetrazolium Salts
Thiazoles
Tissue Donors
Caspase 3
DNA Nucleotidylexotransferase
Indoles
Nitric Oxide
Nitroprusside
Tetrazolium Salts
Thiazoles
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effects of Morphine on Free Radical-induced Apoptosis of Human Neuroblastoma SH-SY5Y Cells
- Protective Effect of PKC and Nitric Oxide Affecting Taxol-Induced Cytotoxicity in C6-Gial Cells
- Function of bcl-X proteins in Nitric Oxide-induced Apoptosis in RAW 264.7 Macrophages
- Effects of Citrus limon Extract on Oxidative Stress-Induced Nitric Oxide Generation and Bovine Teeth Bleaching
- Anti-apoptotic effect of fermented Citrus sunki peel extract on chemical hypoxia-induced neuronal injury
