J Korean Acad Periodontol.  2004 Jun;34(2):317-332. 10.5051/jkape.2004.34.2.317.

Study on the Biological Characteristics of Cultured Osteoblasts Derived from Alveolar Bone

• Lee YB
• Lee SJ
• You SJ
• Kim HA
• You HK
• Shin HS

• KMID: 2045157
• DOI: http://doi.org/10.5051/jkape.2004.34.2.317

Abstract

Osteoblasts from alveolar bone may have an important role in the bone regeneration for periodontium, but their culture and characterization are not determined yet. The purpose of this study was to investigate the biological characteristics of primary explant cultured osteoblasts(PECO) from alveolar bone. Osteoblasts were isolated and cultured from alveolar socket of extracted tooth in children. To compare the characteristics, osteoblasts and gingival fibroblasts were cultured with DMEM at 37degrees C, 5% CO2, 100% humidity incubator, and human fetal osteoblasts cell line(hFOB1) were cultured with DMEM at 34degrees C, 5%, CO2, 100% humidity incubator. To characterize the isolated bone cells, morphologic change, cell proliferation and differentiation were measured. Morphology of PECO was small round body or cuboidal shape on inverted microscope and was similar with hFOB1. PECO became polygonal shape with stellate and had an amorphous shape at 9th passage in culture. PECO had significantly higher activity than that of gingival fibroblasts and hFOB1 in alkaline phosphatase activity. The expression of osteocalcin and bone sialoprotein in PECO was notably increased when compared with hFOB1 and gingival fibroblasts. These result indicated that PECO from alveolar bone in children has an obvious characteristics of osteoblast, may be applied for the regeneration of bone.

Keyword

osteoblast; hFOB1; fibroblast

MeSH Terms

Alkaline Phosphatase
Bone Regeneration
Cell Proliferation
Child
Fibroblasts
Humans
Humidity
Incubators
Integrin-Binding Sialoprotein
Osteoblasts*
Osteocalcin
Periodontium
Population Characteristics*
Regeneration
Tooth
Alkaline Phosphatase
Integrin-Binding Sialoprotein
Osteocalcin