Korean J Lab Med.
2005 Aug;25(4):252-258.
Dissemination of CTX-M Type Extended-Spectrum beta-Lactamases and Emergence of CTX-M-12 in Escherichia coli
- Affiliations
-
- 1Department of Pediatrics, Kosin University College of Medicine, Busan, Korea.
- 2Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea. kscpjsh@ns.kosinmed.or.kr
- 3Department of Yonsei University College of Medicine, Seoul, Korea.
- 4Department of Keonyang University College of Medicine, Daejeon, Korea.
- 5Department of Ajou University College of Medicine, Suwon, Korea.
- 6Department of Hanyang University College of Medicine, Guri, Korea.
- 7Department of Sooncheonhyang University College of Medicine, Gumi, Korea.
- 8Department of Pochon Cha University College of Medicine, Sungnam, Korea.
- 9Department of Chonnam National University Medical School, Gwangju, Korea.
- 10Department of Yonsei University Wonju College of Medicine9, Wonju, Korea.
- 11Department of The Catholic University of Korea College of Medicine10, Seoul, Korea.
- 12Department of Seoul National University College of Medicine11, Seoul, Korea.
- 13Department of Center for Food Safety Evaluation, Seoul, Korea.
- 14Department of Korea Food and Drug Administration12, Seoul, Korea.
Abstract
- BACKGROUND
Clinical isolates of Escherichia coli were evaluated to determine the prevalence and genotypes of Ambler class A extended-spectrum beta -lactamases (ESBLs). METHODS: Clinical isolates of E. coli were collected from 12 hospitals from February through July, 2004. Antimicrobial susceptibility was tested by disk diffusion and agar dilution methods, and ESBLproduction was determined by double-disk synergy test. TEM, SHV, CTX-M, PER-1, VEB, IBC, GES, and TLA type ESBL genes were detected by PCR amplifications, and the PCR products were subjected to direct sequencing.
RESULTS
The double-disk synergy test was positive in 90.9% (149 in 164) of the ceftazidime- or cefotaxime-resistant E. coli isolates. The most prevalent types of Ambler class A ESBLs in E. coliisolates were CTX-M-15 (n=53). CTX-M-14 (n=24), CTX-M-3 (n=9), CTX-M-9 (n=3), CTX-M-12 (n=3), SHV-2a (n=1), SHV-12 (n=5) and TEM-52 (n=3) were also found. CTX-M-12 ESBL had never been reported before in Korea. CONCLUSIONS: CTX-M type ESBL-producing E. coli isolates are spreading and CTX-M-12 is emerging in Korea.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Comparisons of CTX-M-Producing Escherichia coli Isolates from Humans and Animals in South Korea
- The Emerging Strong Among Extended-Spectrum beta-Lactamases: CTX-M Enzymes
- Prevalence of CTX-M Extended-spectrum Beta-lactamases in Clinical Isolates of Enterobacteriaceae in Korea
- In Vitro Susceptibility of piperacillin/tazobactam Against extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae
- Prevalence of the Extended-Spectrum beta-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli
