World J Mens Health.  2015 Aug;33(2):109-116. 10.5534/wjmh.2015.33.2.109.

The Effect of Vitis vinifera L. Juice on Serum Levels of Inhibin B, Sperm Count in Adult Male Rats

Affiliations
  • 1Department of Veterinary, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
  • 2Health Research Institute, Diabetes Research Center, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. akramahangarpour@gm ail.com
  • 3Department of Physiology, Physiology Research Center, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

PURPOSE
Vitis vinifera is a species of Vitis that is native to the Mediterranean region, central Europe, and southwestern Asia, and has been used as a drug in traditional medicine. Traditional medicinal plants have been used for medical purposes with increasing effectiveness. It is important to identify drugs that inhibit spermatogenesis. Therefore, the present study aimed to investigate the effect of grape juice (GJ) on serum levels of inhibin B and sperm count in normal male rats.
MATERIALS AND METHODS
Thirty-five adult male rats were randomly divided into five groups, each containing seven rats. Rats in the control group received 1 mL of normal saline over the course of the study. The experimental groups received GJ (100, 200, 400, and 1,600 mg/kg, orally, for 35 days consecutively). At the end of the treatment period, fertility indices were measured, including body weight difference, sex organ weight, sperm motility and count, epididymal sperm reserve, daily sperm production (DSP), and serum inhibin B levels.
RESULTS
We found that GJ reduces body weight difference, was associated with decreased sperm motility and count in all treatment groups (p< or =0.05 and p< or =0.001, respectively). Moreover, DSP was significantly decreased in all treatment groups compared to the control group (p< or =0.05), except in the group receiving 100 mg/kg of GJ. Inhibin B levels were significantly decreased in all treatment groups (p< or =0.05).
CONCLUSIONS
The results of our study suggest that GJ in all doses, but especially in higher doses, may decrease fertility in male rats.

Keyword

Inhibin B; Male rat; Sperm count

MeSH Terms

Adult*
Animals
Asia
Body Weight
Europe
Fertility
Humans
Inhibins*
Male*
Medicine, Traditional
Mediterranean Region
Organ Size
Plants, Medicinal
Rats*
Sex Characteristics
Sperm Count*
Sperm Motility
Spermatogenesis
Spermatozoa*
Vitis*
Inhibins

Reference

1. Bazrafkan M, Panahi M, Saki G, Ahangarpour A, Zaeimzadeh N. Effect of aqueous extract of ruta graveolens on spermatogenesis of adult rats. Int J Pharmacol. 2010; 6:926–929.
Article
2. Nikkah R, Ebadi A, Naghavi MR, Cresti M, Scali M, Hadadynejad M. Application of SSR markers for characterization of genetic diversity within Iranian grapevine cultivars ('Askari' and 'Keshmeshi'). Hortic Environ Biotechnol. 2010; 51:39–44.
Article
3. Favaron F, Lucchetta M, Odorizzi S, Pais da Cunha AT, Sella L. The role of grape polyphenols on trans-resveratrol activity against Botrytis cinerea and of fungal laccase on the solubility of putative grape PR proteins. J Plant Pathol. 2009; 91:579–588.
4. Leifert WR, Abeywardena MY. Cardioprotective actions of grape polyphenols. Nutr Res. 2008; 28:729–737. PMID: 19083481.
Article
5. Mennen LI, Walker R, Bennetau-Pelissero C, Scalbert A. Risks and safety of polyphenol consumption. Am J Clin Nutr. 2005; 81:326S–329S. PMID: 15640498.
Article
6. Govand AA, Hamid Ghaffoori H, Aso Omer R. Serum levels of male oligospermia glycoconjugate inhibin B hormone and α-L-fucose in Kurdistani (Iraq) populations. IJBAS-IJENS. 2012; 12:59–66.
Article
7. Dadfar M, Ahangarpour A, Habiby A, Khazaely D. Pre-operative serum level of inhibin B as a predictor of spermatogenesis improvement after varicocelectomy. Urol J. 2010; 7:110–114. PMID: 20535698.
Article
8. Razie S, Panahi M, Ahangarpour A, Rahim F, Saki G. Study of level of inhibin B and ultra structure of sertoli cells in contra-lateral testis after unilateral blunt testis trauma in rat. Asian J Biol Sci. 2011; 4:70–76.
9. Ramaswamy S, Marshall GR, Pohl CR, Friedman RL, Plant TM. Inhibitory and stimulatory regulation of testicular inhibin B secretion by luteinizing hormone and follicle-stimulating hormone, respectively, in the rhesus monkey (Macaca mulatta). Endocrinology. 2003; 144:1175–1185. PMID: 12639898.
10. Krause W, Bohring C. Inhibin B as a marker of spermatogenesis. A new dimension in andrology. Hautarzt. 2002; 53:5–10. PMID: 11963224.
11. Buzzard JJ, Loveland KL, O'Bryan MK, O'Connor AE, Bakker M, Hayashi T, et al. Changes in circulating and testicular levels of inhibin A and B and activin A during postnatal development in the rat. Endocrinology. 2004; 145:3532–3541. PMID: 15070852.
12. Hogan S, Zhang L, Li J, Sun S, Canning C, Zhou K. Antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase. Nutr Metab (Lond). 2010; 7:71. PMID: 20799969.
13. Ahangarpour A, Oroojan AA, Heidari H. Effects of exendin-4 on male reproductive parameters of d-galactose induced aging mouse model. World J Mens Health. 2014; 32:176–183. PMID: 25606567.
Article
14. Kaushik MC, Misro MM, Sehgal N, Nandan D. Effect of chronic oestrogen administration on androgen receptor expression in reproductive organs and pituitary of adult male rat. Andrologia. 2010; 42:193–205. PMID: 20500749.
Article
15. Bullock G, Bunton TE. The laboratory rat. In : Krinke GJ, editor. The handbook of experimental animals: the laboratory rat. New York: Academic Press;2000. p. 150.
16. Gilber SF. Developmental biology. 5th ed. Sunderland, MA: Sinauer Associates;1997. p. 755–758.
Article
17. World Health Organization (WHO). WHO protocol MB-50. A method for the examining the effect of a plant extract administration orally on the fertility of male rats (APF/IP, 9914E). Geneva: WHO;1983.
Article
18. Parandin R, Sadeghipour Rodsari HR, Shamili S, Ghasempour HR. Effects of aqueous extract of Boswellia thurifera on fertility in male rats. ZUMS J. 2009; 16:23–30.
Article
19. Al-Daraji HJ. The use of certain grape constituents for improve semen quality and storage ability of diluted Roosters' semen. Am J Pharm Tech Res. 2012; 2:308–322.
Article
20. Castilla P, Echarri R, Dávalos A, Cerrato F, Ortega H, Teruel JL, et al. Concentrated red grape juice exerts antioxidant, hypolipidemic, and antiinflammatory effects in both hemodialysis patients and healthy subjects. Am J Clin Nutr. 2006; 84:252–262. PMID: 16825703.
Article
21. Boqué N, Campión J, de la Iglesia R, de la Garza AL, Milagro FI, San Román B, et al. Screening of polyphenolic plant extracts for anti-obesity properties in Wistar rats. J Sci Food Agric. 2013; 93:1226–1232. PMID: 23080265.
Article
22. Ola M, Al-Sanabra F, Eyad A, Qunaibi Talal A, Aburjai Farouq A, Al-Qaadan Maha S, et al. Antifertility activity of ethanolic seed extract of celery (Apium graveolens L.) in male albino rats. Jordan J Pharm Sci. 2013; 6:30–39.
23. Anderson RA, Sharpe RM. Regulation of inhibin production in the human male and its clinical applications. Int J Androl. 2000; 23:136–144. PMID: 10844538.
24. Illingworth PJ, Groome NP, Byrd W, Rainey WE, McNeilly AS, Mather JP, et al. Inhibin-B: a likely candidate for the physiologically important form of inhibin in men. J Clin Endocrinol Metab. 1996; 81:1321–1325. PMID: 8636325.
Article
25. Piyachaturawat R, Timinkul A, Chuncharunee A, Suksamrarn A. Growth suppressing effect of curcuma comosa extract on male reproductive organs in immature rats. Pharm Biol. 1998; 36:44–49.
Article
26. Yakubu MT, Akanji MA, Oladiji AT. Evaluation of antiandrogenic potentials of aqueous extract of Chromolaena odoratum (L.) K. R. leaves in male rats. Andrologia. 2007; 39:235–243. PMID: 18076423.
Article
27. Andersen ML, Tufik S. Does male sexual behavior require progesterone? Brain Res Rev. 2006; 51:136–143. PMID: 16386800.
28. Srivastav A, Chandra A, Singh M, Jamal F, Rastogi P, Rajendran SM, et al. Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant. Reprod Toxicol. 2010; 29:214–224. PMID: 19903524.
Article
29. Ahangarpour A, Oroojan AA, Heidari H, Ehsan G, Rashidi Nooshabadi MR. Effects of hydro-alcoholic extract of Rhus coriaria (sumac) seeds on reproductive complications of nicotinamide-streptozotocin induced type-2 diabetes in male mice. World J Mens Health. 2014; 32:151–158. PMID: 25606564.
Article
30. Afzalzadeh MR, Amirzargar A, Ahangarpour A, Kazemivarnamkhasti M, Ganjali H, Gharibmombeni E, et al. Effects of Vitis vinifera leave hydro-alcoholic extract on reproductive parameters in adult normal male rats. J Phys Pharm Adv. 2013; 6:159–167.
Article
Full Text Links
  • WJMH
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr