J Korean Rheum Assoc.
1996 Jan;3(1):64-69.
Isolation and Characterization of Human Muscle Cells
- Affiliations
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- 1Institute of Rheumatology, Department of Internal Medicine, Hanyang University, Seoul, Korea.
Abstract
OBJECTIVES
To isolate and culture of human muscle cells by manipulating culture conditions.
METHODS
Muscle samples were obtained during total hip replacement or bedside muscle biopsy. We isolated myoblasts from freshly obtained human muscle tissue by trypsin and collagenase digesion.
RESULTS
Selective isolation of myoblasts was identified through histologic examination by light-microscope. We identified monoc]onal antibodies(Leu-19) as molecular markers in human skeletal muscle were expressed.
CONCLUSIONS
This study demonstrated that it had been possible to culture morphologically and immunelogically identifiable myoblasts isolated from human skeletal muscle tissue.
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