J Korean Radiol Soc.  2007 May;56(5):431-437. 10.3348/jkrs.2007.56.5.431.

Various Ocular MR Imagings in a Mouse Implanted with a New Cell Line of Retinoblastoma and the Correlation with the Pathology: Preliminary Study

• Kim DH
• Kim IJ
• Yang JH
• Byun JN
• Kim JH
• Yu YS
• Lee BJ

• KMID: 2024506
• DOI: http://doi.org/10.3348/jkrs.2007.56.5.431

Abstract

PURPOSE
We wanted to show various MR and correlated pathologic images of retinoblastoma in nude mousewith a new human retinoblastom cell line (SNUOT-Rb1), which was inoculated into the intravitreous cavity.
MATERIALS AND METHODS
The established cell line was inoculated into the intravitreous cavity of 36 eyeballs of 18 mice and the transplanted retinoblastoma was examined for 3 months. The T1-weighted (T1WI), T2-weighted (T2WI), and contrast enhanced (Gd-DTPA) T1-weighted images were obtained with using a small loop coil. After scanning, the mice's eyeballs were extracted and the hematoxylin & eosin stained specimens were examined with a microscope. We compared the MR imagings with pathologic findings and evaluated the character of the tumors.
RESULTS
The innoculated cells in the eyeballs of the mice grew into retinoblastoma (23/36, 64%). The eyeballs with retinoblastoma protruded externally and showed focal hemorrhage. Most tumors showed iso-signal intensity on TIWI (13/23, 57%), high signal intensity on T2WI (17/23, 74%), and good enhancement (21/23, 91%) with contrast. Almost all of the tumors (n=21) were located in the retina and three extraretinal tumors were confirmed by pathology. Involvement of the optic nerve was suspected on MRI and this was confirmed by pathology in 6 cases and 5 cases, respectively.
CONCLUSION
We could demonstrate various MR imagings of transplanted retinoblastoma by using the new tumor cell line in vivo.

Keyword

Eye, MR; Eye, neoplasms; Retina, neoplasms; Animals

MeSH Terms

Animals
Cell Line*
Cell Line, Tumor
Eosine Yellowish-(YS)
Hematoxylin
Hemorrhage
Humans
Magnetic Resonance Imaging
Mice*
Optic Nerve
Pathology*
Retina
Retinoblastoma*
Eosine Yellowish-(YS)
Hematoxylin