J Korean Orthop Res Soc.
2003 Apr;6(1):117-126.
Cellular Biocompatibility and Stimulatory Effects of Calcium Metaphosphate on Osteoblastic Differentiation of Human Bone Marrow-derived Stromal Cells
- Affiliations
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- 1Biomedical Research Institute, Kyungpook National University Hospital, Korea.
- 2Department of Biochemistry, School of Medicine, Kyungpook National University, Korea. syukim@knu.ac.kr e-mail
- 3Department of Oral Pathology, College of Dentistry, Kyungpook National University, Korea.
- 4School of Materials Engineering, Yeungnam University, Korea.
- 5Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University
Abstract
- PURPOSE
The in vitro biocompatibility of Calcium Metaphosphate (CMP) with human bone marrow stromal cells (HBMSCs) and its effect on osteoblastic differentiation have been evaluated. MATERIALS AND METHODS: The effects of CMP on the HBMSCs undergoing osteoblastic differentiation were evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Morphologies of the HBMSCs were examined using scanning electron microscopy and confocal laser scanning microscopy. Osteoblastic differentiation of the HBMSCs was analyzed by alkaline phosphatase (ALP) staining and RTPCR. RESULTS: The CMP powder and disk did not exert cytotoxic effect on the HBMSCs. In addition, the HBMSCs were adhered on the surface of CMP disk as successfully as on the culture plate or HA disk and displayed similar actin arrangement and cellular phenotypes. Furthermore, the HBMSCs grown on three different matrices were able to support osteoblastic differentiation of the HBMSCs as accessed by ALP staining. However, the CMP disk compared to the HA disk has a better ability to induce expression of osteoblast-related genes such as ALP, osteopontin (OPN) and osteoprotegerin (OPG). CONCLUSION: The results demonstrate that, in addition to biocompatibility of the CMP with the HBMSCs, the CMP has an ability to stimulate osteoblastic differentiation of the HBMSCs in vitro.
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