J Korean Acad Periodontol.  2005 Dec;35(4):1097-1108. 10.5051/jkape.2005.35.4.1097.

Osteoblastic differentiation of adult stem cells by Biphasic Calcium Phosphate

• Lee MK
• Lim SW
• Pi SH
• Kim YS
• Shin HS
• You HK

• KMID: 1999715
• DOI: http://doi.org/10.5051/jkape.2005.35.4.1097

Abstract

The present study was to determine the influence of micro-macro biphasic calcium phosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that MBCP may stimulates the osteoblastic activity of ASC.

Keyword

Osteoblastic differentiation; mesenchymal stem cells; biphasic calcium phosphate(MBCP)

MeSH Terms

Adult Stem Cells*
Adult*
Alkaline Phosphatase
Blotting, Western
Bone Marrow
Calcium*
Cell Count
Cell Proliferation
Collagen
Humans
Integrin-Binding Sialoprotein
Mesenchymal Stromal Cells
Osteoblasts*
Osteocalcin
Stem Cells
Alkaline Phosphatase
Calcium
Collagen
Integrin-Binding Sialoprotein
Osteocalcin