J Korean Orthop Assoc.  2001 Dec;36(6):641-647.

In Vitro Bone Formation by Osteoblasts in Calcium Metaphosphate and Highly Purified Bovine Xenograft (Lubboc(R))

• Ahn MW
• Jang JH
• Park SK
• Kim SD
• Jeong JH

Abstract

PURPOSE
This study was performed to compare osteogenic capacity according to the scaffold, i.e., calcium metaphosphate on purified bovine collagen (Lubboc(R)).
MATERIALS AND METHODS
Calcium metaphosphate and Lubboc blocks were immersed within a bone marrow cell suspension from New Zealand white male rabbit anterior superior iliac spine to produce cell-biomaterial complexes. These were cultured for 5 weeks in osteogenic media and differences with bone densitometry, light microscopic examination, electron microscopic examination, concentration of osteocalcin from osteoblasts were then evaluated.
RESULTS
In light microscopic examination, more organized tissue was observed in the Lubboc(R) group. By electron microscopy osteoblasts and the matrix were densely overlapped on the surface of the Lubboc(R) group, whereas cells were scattered on the calcium metaphosphate. Immunohistologic examination for osteocalcin was definitely positive in the Lubboc(R) group, but the reaction on the calcium metaphosphate was unclear, and densitometry revealed that Lubboc(R) (0.028 gms/cm2) showed a statistically significant higher density than calcium metaphosphate (0.026 gms/cm2).
CONCLUSION
Highly purified bovine collagen was more effective than calcium metaphosphate in osteogenesis in vitro.

Keyword

Bioceramic; Highly purified bovine collagen (Lubboc(R)); Osteogenesis

MeSH Terms

Bone Marrow Cells
Calcium*
Collagen
Densitometry
Heterografts*
Humans
Male
Microscopy, Electron
New Zealand
Osteoblasts*
Osteocalcin
Osteogenesis*
Spine
Calcium
Collagen
Osteocalcin