Korean J Otolaryngol-Head Neck Surg.  2000 Jun;43(6):577-581.

Neurotoxic Effect of Streptozotocin and Neuroprotective Effect of Insulin Growth Factor-II to the Cultured Mouse Schwann Cells

• Jang CH
• Park ST

Abstract

BACKGROUND: The sensorineural hearing loss due to diabetes is progressive and bilateral, and predominantly occurs in the old, although its accurate pathogenesis is still unknown. Objectives: The purpose of this study is to clarify the neurotoxic effect of streptozotocin (STZ) and the neuroprotective effect of insulin-like growth factor-II(IGF-II) on the cultured Schwann cells of cohlear nerve.
MATERIALS AND METHODS
MTT assays were performed on cultured mouse Schwann cells which were treated with various concentrations of STZ for 24 hours, and the neuroprotective effect of IGF-II against STZ-induced neurotoxicity were also examined.
RESULTS
1) MTT50 value was the concentration of 40 pM STZ (highly toxic : MTT50<100 pM), 2) Cell viability of cultured mouse Schwann cells treated with STZ markedly decreased in a dose-dependent manner.
CONCLUSION
It is suggested that STZ induces a severe toxic effect on cultured Schwann cells of mouse, and selective neurotrophic factors such as IGF-II are very effective in preventing the neurotoxicity induced by STZ.

Keyword

Cultured Schwann cells; Streptozotocin; IGF-II

MeSH Terms

Animals
Cell Survival
Hearing Loss, Sensorineural
Insulin*
Insulin-Like Growth Factor II
Mice*
Nerve Growth Factors
Neuroprotective Agents*
Schwann Cells*
Streptozocin*
Insulin
Insulin-Like Growth Factor II
Nerve Growth Factors
Neuroprotective Agents
Streptozocin