Korean J Phys Anthropol.  1996 Jun;9(1):109-116. 10.11637/kjpa.1996.9.1.109.

Study on the Effect of Vitamin E Against Methylmercury-induced Neurotoxicity in Cultured Spinal Motor Neurons

Abstract

In order to investigate the neurotoxic effect of methylmercury (MM) on cultured mouse spinal motoneuron cell line, NSC-19, neurotoxic effect of MM was evaluated by MTT assay after neurons were incubated with various concentrations of methylmercuric chloride (MMC) for 24 hours. In addition, neuroprotective effect of vitamin E against MMC-induced neurotoxicity was examined by MTT assay and neurofilament enzymeimmunoassay (EIA) in these cultures. The results were as follows : 1. MTT50 value was a concentration of 20µM methylmercuric chloride. 2. Methylmercuric chloride was toxic on cultured spinal motor neurons, NSC-19 cells in a time-and dose-dependent manner by severe decrease of cell viability. 3. Methylmercuric chloride induced the decrease of cell number and the loss of neuritis on these cultures. 4. Vitamin E remarkably increased the viabilily of cultured neurons damaged by methylmercury-induced neurotoxicity at a concentration of 250µM vitamin E. From above the results, it is suggested that methylmercury induces severe toxic effect on cultured mouse spinal motor neurons, NSC-19 cells, and the selective antioxidants such as vitamin E are effective in the neurotoxicity induced by methylmercury in these cultures.

Keyword

NSC-19; Antioxidant; Cell culture; Methylmercury

MeSH Terms

Animals
Antioxidants
Cell Count
Cell Culture Techniques
Cell Line
Cell Survival
Intermediate Filaments
Mice
Motor Neurons*
Neuritis
Neurons
Neuroprotective Agents
Vitamin E*
Vitamins*
Antioxidants
Neuroprotective Agents
Vitamin E
Vitamins
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