Korean J Phys Anthropol.  1996 Dec;9(2):183-188. 10.11637/kjpa.1996.9.2.183.

Effect of Allopurinol on Methylmercuric Chloride in Cultured O1igodendrocytes

• Park ST
• Park BR
• Chung YT

• KMID: 2376318
• DOI: http://doi.org/10.11637/kjpa.1996.9.2.183

Abstract

To elucidate the neurotoxic mechanism of methylmercury on cultured bovine oligodendrocytes, neurotoxic effect was estimated by MTT assay after cultures were exposed to various concentrations of methylmercuric chloride (MMC). In addition, neuroprotective effect of antioxidant, allopurinol agonist MMC-induced neurotoxicity was examined on these cultures. Exposure of cultured bovine oligodendrocytes to MMC showed less than 50% of the cell viability 24 hours after treatment with 35µM of MMC. And also, allopurinol blocked the neurotoxicity induced by MMC on these cultures. These results suggest that oxygen radicals involve in MMC-mediated neurotoxicity, and also seletive antioxidants such as allopurinol are effective in blocking the neurotoxicity induced by MMC on cultured bovine oligodendrocytes.

Keyword

Methylmercury; Cultured bovine oligodendrocyte; Antioxidant; Oxygen radicals

MeSH Terms

Allopurinol*
Antioxidants
Cell Survival
Neuroprotective Agents
Oligodendroglia
Reactive Oxygen Species
Allopurinol
Antioxidants
Neuroprotective Agents
Reactive Oxygen Species