J Korean Diabetes Assoc.
2002 Dec;26(6):451-459.
Effect of Mouse Type and Human Type of CpG Oligonucleotide Vaccination on Development of Diabetes in NOD Mice
- Affiliations
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- 1Department of Endocrinology and Metabolism, Wonju College of Medicine, Yonsei University, Wonju, Korea.
- 2Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju, Korea.
- 3The Institute of Functional Biomaterials and Biotechnology, Yonsei University, Korea.
- 4Department of Pathology, Chung-Ang Medical College, Chung-Ang University, Seoul, Korea.
- 5Department of Internal medicine, Konkuk Medical college, Konkuk University, Seoul, Korea.
Abstract
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BACKGROUND: Type 1 diabetes is autoimmune disease and the modulation of immune system could offer breakthrough to the disease. Unmethylated CpG motifs and their oligoneucleotide are potent immunostimulators that can rebalance autoimmune mechanism. To explore DNA based immunotherapy in type 1 diabetes, we vaccinated different types (mouse and human) of CpG ODN to NOD mice.
METHODS
Forty 5 week-old female NOD mice were injected with 100 L (10 g) of mouse type CpG ODN or human type CpG ODN or 0.9% normal saline on inguinal area subcutaneously. Seven, 14, and 28 days later we injected to mice same dose of mouse type CpG ODN or human type CpG ODN or normal saline. Blood glucose was measured and mice were sacrificed when they were diabetic. Pancreata and serum were earned from sacrificed NOD mice to evaluate insulitis and insulin immunoassay.
RESULTS
Though the final cumulative incidences of diabetes were not significantly different among groups, the tendency of delaying and suppressing the development of diabetes was observed in the early period of vaccination group of CpG ODN. Especially, mouse type CpG ODN was more effective for rodent species than human type CpG ODN.
CONCLUSION
This result suggests that immunomodulation therapy using species- specific CpG motif may have a potential to control autoimmune process as well as dissecting T cell milieu in NOD mice.
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