J Korean Surg Soc.  1997 Aug;53(2):153-161.

Changes in the Fxpression of DNA Synthesis According to Time Hepatocyte Growth Factor, Transforming Growth Factor-beta1 mRNA and c-met mRNA in Regenerating Rat Liver after Partial Hepatectomy during 24 Hours

• Kim WH
• Cho HS
• Lee JH
• Park HL
• Wang HJ
• Kim MW

Abstract

The capacity of the liver to regenerate after a reduction in liver mass induced by surgical removal is quite remarkable. The most widely studied example of this phenomenon is accompanied by many investigators but the exact mechanism is not clear. Over the past decade, it has been demonstrated that growth factors play a major role in liver regeneration. Several growth factors such as epidermal growth factor, transforming growth factor(TGF), acidic fibroblast growth factor and hepatocyte growth factor(HGF) are involved in liver regeneration. Recently, HGF has aroused increasing interest due to its powerful mitogenic effect and multiple functions on various cell types. The receptor for HGF has recently been characterized as the product of the protooncogene c-met. We have examined the changes of synthesis of DNA and the expression of HGF mRNA and c-met mRNA that be involved in liver regeneration, after partial hepatectomy in the rat. Also we have examined the expression of TGF- mRNA that might be involved in inhibition of epithelial cell regeneration and differentiation of hepatocyte. Proliferative index was determined to use in vivo thymidine uptake. Nothern blot analysis used to assay HGF mRNA an TGF- mRNA. RNase protection assay used to assay c-met mRNA. The thymidine uptake of the remnant liver was abruptly increased after 18 hours, was maintained 24 hours. The nothern blot analysis showed that the expression of HGF mRNA and TGF- mRNA increased continuously within 24 hours. The RNase protection assay showed that the expression of c-met mRNA decreased continuously during one day after partial hepatectomy. The data presented here suggests that HGF is involved in liver regeneration and continuous decrease of c-met mRNA is phenomenon of receptor down-regulation. The increase of TGF- mRNA, well known as inhibitor of cell regeneration, suggests that TGF- is control mechanism of liver regeneration.

Keyword

Partial Hepatectomy; HGF mRNA; c-met mRNA; TGF-beta1 mRNA

MeSH Terms

Animals
DNA*
Down-Regulation
Epidermal Growth Factor
Epithelial Cells
Fibroblast Growth Factor 1
Hepatectomy*
Hepatocyte Growth Factor*
Hepatocytes*
Humans
Intercellular Signaling Peptides and Proteins
Liver Regeneration
Liver*
Rats*
Regeneration
Research Personnel
Ribonucleases
RNA, Messenger*
Thymidine
DNA
Epidermal Growth Factor
Fibroblast Growth Factor 1
Hepatocyte Growth Factor
Intercellular Signaling Peptides and Proteins
RNA, Messenger
Ribonucleases
Thymidine