J Korean Soc Spine Surg.  2002 Sep;9(3):172-177. 10.4184/jkss.2002.9.3.172.

Immuno-expression of Type IV Collagen in the Intervertebral Disc

• Jeon CH
• Moon JH
• Kang YH

• KMID: 2003221
• DOI: http://doi.org/10.4184/jkss.2002.9.3.172

Abstract

STUDY DESIGN: In vitro studies using human intervertebral disc for the localization of the type IV collagen.
OBJECTIVE
1) To study the distribution pattern and immunoexpression of type 4 collagen in the intervertebral disc, 2) To study the function of type IV collagen in the intervertebral disc. SUMMARY OF BACK GROUND: The correlations of degeneration changes and collagens in the dics have not been determined. The reports for type IV collagen were few. So far, the histologic analysis for the expression of type IV collagen in the intervertebral disc has not been done. There was no report to study the function of the type IV collagen in the intervertebral disc.
METHODS
Fifty-four disc blocks obtained during anterior interbody fusion of the lumbar spine were used to observe the expression pattern of the type IV collagen with immunochemical stain. For the observation of the myxomatous degeneration in the intervertebral disc, the alcian blue stain with periodic acid-schilff was done. For the control group, 22 neonate intervertebral disc blocks were obtained at autopsy.
RESULTS
The immunoreactions for type IV collagen were associated blood vessels in the anulus fibrosus in the disc. There was no statistical significant difference of the type IV collagen expression between the control and disease groups. Myxomatous degenerations were observed as the irregular form in the degenerative intervertebral disc.
CONCLUSION
The immunoreactions for the type IV collagen were observed in the intervertebral discs and associated with the formation of the blood vessels, especially in anulus fibrosus.

Keyword

Intervertebral disc; Type IV collagen; Blood vessel

MeSH Terms

Alcian Blue
Autopsy
Blood Vessels
Collagen
Collagen Type IV*
Dacarbazine
Humans
Infant, Newborn
Intervertebral Disc Degeneration
Intervertebral Disc*
Spine
Alcian Blue
Collagen
Collagen Type IV
Dacarbazine

Figure

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  Fig. 1. The myxomatous degenerations(*) in the degenerative intervertebral disc were observed in irregular form (×200).

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  Fig. 2. Extracellular matrix fibers were seen with regular form(*) in the neonate disc (× 200).

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  Fig. 3. Blood vessels associated with the type IV collagen was abserved in anulus fibrosus (× 100).

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  Fig. 4. Type IV Collagen was expressed with blood vessels in neonate disc (× 200).

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