J Korean Acad Prosthodont.  2005 Jun;43(3):393-408.

Gene-expression profiling of titanium-cell interaction

• Kim CS
• Hwang JW
• Ryu JJ
• Shin SW
• Sohn SH
• Kim KN
• Kim MK

Abstract

STATEMENT OF PROBLEM: In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function. PURPOSE: This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63). MATERIALS AND METHODS: MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit and acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements). RESULTS: The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces. CONCLUSION: The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.

Keyword

Titanium surfaces; expression profiling; cDNA microarray; MG63

MeSH Terms

Bone Morphogenetic Proteins
Cell Line
Collagen
Dental Materials
DNA, Complementary
Genes, Regulator
Humans
Oligonucleotide Array Sequence Analysis
Osseointegration
Osteoblasts
Osteogenesis
Receptor, Fibroblast Growth Factor, Type 4
RNA
Somatomedins
Titanium
Bone Morphogenetic Proteins
Collagen
DNA, Complementary
Dental Materials
RNA
Receptor, Fibroblast Growth Factor, Type 4
Somatomedins
Titanium