J Korean Acad Periodontol.  2007 Dec;37(4):743-753. 10.5051/jkape.2007.37.4.743.

The Gingival Tissue Levels of Bone Resorptive Mediators in Human Chronic Periodontitis with Type 2 Diabetes Mellitus

• Kim MJ
• Ryu SH
• Park JW
• Suh JY
• Lee JM

• KMID: 1999615
• DOI: http://doi.org/10.5051/jkape.2007.37.4.743

Abstract

PURPOSE
The purposes of this study were to compare and quantify the expression of MMP-3, PGE2 and IL-6 in the gingival tissues of patients with type2 diabetes mellitus and healthy adults with chronic periodontitis. MATERIAL AND METHODS: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1(n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2(n=8) is inflamed gingiva from patients with chronic periodontitis. Group 3(n=8) is inflamed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Westernblotting. The quantification of MMP-3, PGE2 and IL-6 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test.
RESULTS
1. The expression levels of MMP-3 were shown highest in group 3 compared to group 1 and 2, and It showed increasing tendency in group 2 and 3. 2. The expressions of PGE2 and IL-6 were shown increasing tendency in group 2 and 3, and It was highest in group 3. 3. As expressions of MMP-3 were increased, PGE2 and IL-6 expressions showed increasing tendency in group 3 than group1 and 2, although there were no proportional relationship.
CONCLUSION
This study demonstrated that the expression levels of MMP-3, PGE2 and IL-6 will be inflammatory markers of periodonta linflamed tissue and DM. It can be assumed that MMP-3 affect to expressions of PGE2 and IL-6 in progression of periodontal inflammation with alveolar bone resorption to type 2 DM.

Keyword

Chronic periodontitis; diabetes mellitus; bone resorption factor

MeSH Terms

Adult
Bone Resorption
Chronic Periodontitis*
Diabetes Mellitus
Diabetes Mellitus, Type 2*
Dinoprostone
Gingiva
Hemorrhage
Humans*
Inflammation
Interleukin-6
Periodontal Pocket
Tooth Extraction
Dinoprostone
Interleukin-6