Korean J Obstet Gynecol.  2007 Feb;50(2):280-287.

Changes of interleukin-6, tumor necrosis factor-alpha, and total antioxidant ability in the umbilical venous plasma of preterm birth with or without premature rupture of membranes

• Jeong KP
• Kim YH
• Kim CH
• Cho MK
• Kim KM
• Kim JW
• Ahn BW
• Yang SY
• Joo EH
• Song TB

Abstract

OBJECTIVE
To compare interleukin-6 (IL-6), tumor necrosis factor-alpha(TNF-alpha), oxygen-radical absorbance capacity (ORAC), and antioxidant vitamin levels in the umbilical venous plasma of preterm labor with intact membranes (PTL) and preterm premature rupture of membrane (PPROM) with that of full term normal pregnancy (NP) and to evaluate their roles of pathophysiology in preterm labor and PPROM.
METHODS
Umbilical venous blood samples were collected from women with PTL (n=30), PPROM (n=30) and NP (n=30). IL-6 and TNF-alpha were measured by enzyme-linked immunosorbent assay. Lipid peroxide levels were measured by thiobarbituric acid reaction. The ORAC was mesured by Cao's method. Antioxidant vitamin levels were measured by high performance liquid chromatography.
RESULTS
IL-6 and TNF-alpha levels in the umbilical venous plasma of PPROM were significantly higher than that of PTL and NP (3.28+/-0.31 vs. 2.84+/-0.19 vs. 2.79+/-0.22 pg/ml, p<0.05), (2.30+/-0.27 vs. 1.64+/-0.23 vs. 1.40+/-0.25 pg/ml, p<0.01). Lipid peroxide levels in the umbilical venous plasma of PPROM were significantly higher than that of NP and PTL (2.78+/-0.27 vs. 2.54+/-0.32 vs. 2.24+/-0.24 nmol/mg protein, p<0.01). ORAC levels in the umbilical venous plasma of PTL and PPROM were significantly lower than that of NP (967425.4+/-98.99 vs. 965165.8+/-91.20 vs. 1011328.5+/-85.96 U/ml, p<0.05). Ascorbic acid levels in the umbilical venous plasma of PPROM were significantly lower than that of NP and PTL (125.6+/-2.33 vs. 158.3+/-3.08 vs. 221.7+/-2.82 nmol/ml, p<0.01).
CONCLUSION
Increase in inflammatory cytokines (IL-6, TNF-alpha) and imbalance of increased lipid peroxidation and decreased antioxidant activity in umbilical venous blood may be involved in the pathophysiology of PPROM.

Keyword

IL-6; TNF-alpha; Total antioxidant ability; Umbilical venous plasma; Preterm birth

MeSH Terms

Ascorbic Acid
Chromatography, Liquid
Cytokines
Enzyme-Linked Immunosorbent Assay
Female
Humans
Interleukin-6*
Lipid Peroxidation
Membranes*
Necrosis
Obstetric Labor, Premature
Plasma*
Pregnancy
Premature Birth*
Rupture*
Tumor Necrosis Factor-alpha*
Vitamins
Ascorbic Acid
Cytokines
Interleukin-6
Tumor Necrosis Factor-alpha
Vitamins