PCR-based Study on Loss of Heterozygosity at the Short Arm of Chromosome 3 in Renal Cell Carcinoma

Yoon, Jin Han; Park, Young Ho; Kim, In Hoo

Abstract

Loss of heterozygosity (LOH) at polymorphic loci on the short arm of chromosome 3 which has multiple recurrence genes was investigated in histopathologically proven 35 sporadic renal cell carcinoma (RCC) at Dong-A University. The allelic frequencies and the rate of LOH applying a polymerase chain reaction (PCR) at five loci, D3S2 (MSPI), THRB (EcoRI), THRB (BamHI), THRB (MSPI) and D3F15S2 (Hind III) were investigated. The allelic frequencies were quite different between normal subjects and patients with RCC in Koreans. The rate of LOH was shown lower at all loci than those of Caucasians. The clear distinction in the incidence of LOH was shown between clear cell and granular cell type. LOH was not observed at THRB locus in clear cell type whereas in granular cell type, LOH could not be observed at D3Fl5S2 locus. The following conclusion was derived. The author found that one allelic gene was changed in patients with RCC because the allelic frequencies were different between normal subjects and patients with RCC. Also, the genetic characteristics in Koreans were different from Caucasians, and the detection of genetic loci specific for Koreans is important in oncogenetic studies. Lastly, Allelic analysis was compared with the histopathological findings of genetic alterations so to correlate the relationship between oncogenesis and genetic alterations. Therefore, this study suggested that different genes are involved in oncogenesis according to histopathological types.