Korean J Med.
2000 Aug;59(2):151-160.
Relation of gastric epithelial apoptosis with CagA, VacA, and ammonia produced by Helicobacter pylori
- Affiliations
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- 1Department of Internal Medicine, Soon Chun Hyang University College of Medicine, Seoul, Korea.
- 2Department of Pathology, Soon Chun Hyang University College of Medicine, Seoul, Korea.
- 3Institute for Digestive Research, Soon Chun Hyang University College of Medicine, Seoul, Korea.
Abstract
- BACKGROUND
H. pylori infection is associated with increased apoptosis of gastric epithelium. However, the mechanism remains unclear what product of the H. pylori causes apoptosis. This study was to investigate the roles of CagA, VacA protein and ammonia produced by H. pylori in inducing the apoptosis of gastric epithelium.
METHODS
Endoscopic biopsy samples were taken for rapid urease test, histology, and culture in 34 patients with peptic ulcer disease. And we analysed the ammonia level of gastric juice and CagA and VacA protein in serum. Apoptosis in situ was examined by using the methods of terminal uridine deoxynucleotidyl nick end-labelling (TUNEL). All patients infected by H. pylori were treated with antibiotics, and reevaluated the above studies after treatment.
RESULTS
A total of 34 patients with peptic ulcer disease were enrolled: 10 were uninfected and 24 patients were infected with H. pylori. Of the 24 infected patients, 11 harbored CagA+VacA+ strains and 10 were infected with CagA+VacA- strains. Three patients were infected with CagA-VacA- strains. The ammonia level of gastric juice in infected patients by H. pylori was significantly higher than that in uninfected and posteradication therapy (92.3+/-52.3 mM vs. 13.8+/-6.1 mM and 16.8+/-11.8 mM). Apoptosis indices in infected patients by H. pylori were higher than those in uninfected and posteradication therapy (12.3+/-3.4 vs. 7.6+/-2.2 and 8.2+/-3.8). The difference in apoptotic indices between the patients haboring CagA+VacA+strains (n=11) and CagA+VacA- strains (n=10) was not statistically significant (12.6+/-3.4 vs. 12.3+/-3.1). There was a positive correlation between apoptotic indices and ammonia concentration in the gastric juice (r=0.376, p<0.05).
CONCLUSION
These results indicate that H. pylori infection and increased production of ammonia in gastric juice may induce gastric epithelial apoptosis.
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