Cancer Res Treat.  2003 Dec;35(6):489-496.

Proteomic Profiling of Human Small Cell Lung Cancer Cell Line NCI-H211

• Cho HY
• Kim MK
• Yoo YD
• Ahn MJ
• Jang JS

Abstract

PURPOSE
Small cell lung cancer is one of the major causes of death from cancer worldwide. To explore the expressions of global protein in small cell lung cancer cells, a proteomic approach, to identify the proteins, was used and the establishment of a protein reference map attempted. MATERIALS AND METHODS: Two-dimensional gel electrophoresis (2-DE), with subsequent analysis by mass spectrometry (MS), was applied to the study of protein identification from a small cell lung cancer cell line, NCI- H211. The cells were lysed, and the extracts subjected to isoelectric focusing, with immobilized pH gradients, followed by second dimension SDS-PAGE. The polypeptides were identified by peptide mass fingerprinting, with MALDI-TOF MS, after in-gel protein digestion. RESULTS: From silver staining of the gel, around two thousands protein spots were separated by the 2-DE. Of these protein spots visualized in the gel, one hundred and ten were identified by peptide mass fingerprinting. Different proteins, such as enzymes, cytoskeletal proteins and proteins common to eukaryotic cells, were identified. CONCLUSION: The protein expressions of the small cell lung cancer cells were analyzed to establish a protein reference map. The reference map presented here may serve as a working tool for the further study of small cell lung cancer.

Keyword

Proteome; Small cell lung cancer; Reference map

MeSH Terms

Cause of Death
Cell Line*
Cytoskeletal Proteins
Dermatoglyphics
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Eukaryotic Cells
Humans*
Isoelectric Focusing
Mass Spectrometry
Peptides
Proteolysis
Proteome
Proton-Motive Force
Silver Staining
Small Cell Lung Carcinoma*
Cytoskeletal Proteins
Peptides
Proteome