Tuberc Respir Dis.  2003 Jul;55(1):88-97. 10.4046/trd.2003.55.1.88.

Genetic Polymorphism of Epoxide Hydrolase and GSTM1 in Chronic Obstructive Pulmonary Disease

• Park SS
• Kim EJ
• Son CY
• Wi JO
• Park KH
• Cho GJ
• Ju JY
• Kim KS
• Kim YI
• Lim SC
• Kim YC
• Park KO
• Na KJ

• KMID: 1970547
• DOI: http://doi.org/10.4046/trd.2003.55.1.88

Abstract

BACKGROUND: Although smoking is a major cause of chronic obstructive pulmonary disease (COPD), only 10-20% of cigarette smokers develop symptomatic COPD, which suggests the presence of genetic susceptibility. This genetic susceptibility to COPD might depend on variations in the activities of the enzyme that detoxify hazardous chemical products, such as microsomal epoxide hydrolase (mEPHX) and glutathione-S transferase M1 subunit (GSTM1) genes.
METHODS
The genotypes of 58 patients with COPD, and 79 age matched control subjects, were determined by a polymerase chain reaction, followed by restriction fragment length polymorphism (PCR-RFLP) for the mEPHX, and multiplex PCR for the GSTM1.
RESULTS
GSTM1 was deleted in 53.3% of the subjects. There was no difference in GSTM1 deletion rates between the COPD patients (32/58, 55.2%) and the control subjects (41/79, 51.9%). The combination patterns of two polymorphisms of mEPHX showed slow enzyme activity in 29(21.2%), normal in 73(53.3%) and fast in 32(23.4%). The COPD group (7/57, 12.3%) showed a significantly lower incidence of slow enzyme activity compared to the control subjects (22/77, 28.6%, p<0.05). However, when the COPD and control groups were compared with smokers only, there were no significant differences in the genotypes of GSTM1 and mEPHX.
CONCLUSION
The genotypes of GSTM1 and mEPHX were not significant risk factors of COPD in this cohort of study.

Keyword

COPD; mEPHX; GSTM1

MeSH Terms

Cohort Studies
Epoxide Hydrolases
Genetic Predisposition to Disease
Genotype
Humans
Incidence
Multiplex Polymerase Chain Reaction
Polymerase Chain Reaction
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length
Pulmonary Disease, Chronic Obstructive*
Risk Factors
Smoke
Smoking
Tobacco Products
Transferases
Epoxide Hydrolases
Smoke
Transferases