J Korean Child Neurol Soc.  1998 Oct;6(1):29-38.

Changes in Gene Expression during Brain Ischemia and the Effect of Pretreatment of BN52021 (PAF Antagonist)

• Nam SO
• Kim SY
• Jung JS

Abstract

BACKGROUND: This study was undertaken to determine temporal changes in expression of various genes and the effect of platelet-activating factor antagonist on their expression.
SUBJECTS AND METHODS
The changes in expression of various genes including interleukin-1, iNOS, TNF-alpha, ICAM-1, cPLA2, GLUT1, BDNF and Bcl-X according to time were examined using reverse-transcription polymerase chain reaction(RT-PCR) by checking at various time points after induction of middle cerebral artery(MCA) occlusion in the thrombotic and embolic models of stroke of Sprague-Dawley rats. We also examined the effect of pretreatment of a PAF antagonist, BN52021 on the expression of the same genes by RT-PCR and by in situ hybridization technique.
RESULTS
The expressions of BDNF, IL-1, GLUT1, iNOS, cPLA2 and TNF-alpha were increased in ischemic brain tissue. However, the temporal profiles of their expression were variable; the peak expression was observed after 1 hour reperfusion in TNF-alpha, after 4 hours in IL-1, cPLA2 and BDNF and after 24 hours in GLUT1 and iNOS. Pretreatment of a PAF antagonist, BN52021, significantly inhibited the ischemia-induced expression of TNF-alpha, cPLA2 and iNOS without affecting the expression of the BDNF and GLUT1. In situ hybridization showed that cPLA2 expression induced by transient forebrain ischemia in dentate gyrus was ameliorated by the pretreatment of BN52021.
CONCLUSION
This result indicates that BN52021, PAF antagonist, specifically inhibits expression of certain genes, which may be related to its protective effect on ischemic brain injury.

Keyword

Brain ischemia; Gene expression; PAF antagonist

MeSH Terms

Brain Injuries
Brain Ischemia*
Brain*
Brain-Derived Neurotrophic Factor
Dentate Gyrus
Gene Expression*
In Situ Hybridization
Intercellular Adhesion Molecule-1
Interleukin-1
Ischemia
Prosencephalon
Rats, Sprague-Dawley
Reperfusion
Stroke
Tumor Necrosis Factor-alpha
Brain-Derived Neurotrophic Factor
Intercellular Adhesion Molecule-1
Interleukin-1
Tumor Necrosis Factor-alpha