J Korean Soc Vasc Surg.
2002 Nov;18(2):251-258.
Measurement of Smooth Muscle Cell Migration on the Micromachined Groove Topology
- Affiliations
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- 1Institute of Advanced Machinery and Design, Seoul National University, Korea. jkchang@snu.ac.kr
- 2Chemical and Biomolecular Engineering, Korean Advanced Institue of Science and Technology, Korea.
- 3Interdisciplinary Program in Medical and Biological Engineering Major, Seoul National University, Korea.
- 4School of Mechanical and Aerospace Engineering, Seoul National University, Korea.
- 5Division of Vascular Surgery, Samsung Medical Center, College of Medicine, Sungkyunkwan University, Korea.
- 6School of Electrical Engineering and Computer Science, Seoul National University, Korea.
- 7Digital Bio Technology Co., Ltd.
Abstract
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PURPOSE: The spreading, orientation, and chemotaxis with the gradient of a chemoattractant of smooth muscle cells (SMCs) were studied on the micro-grooved substrata by the light, fluorescence and scanning electron microscopy.
METHOD: Vertical-walled grooves were produced in silicon wafers by the micromachining technique. All grooves were 4~20micrometer deep and 10~80 micrometer wide. SMCs were cultured on each microgroove and examined under stereo-microscope.
RESULT: Cell clusters were markedly oriented by all the grooved substrata examined. Time-lapse images acquired from CCD (Charge Coupled Device) showed that the grooves directed the migration of SMCs. There was no prominent difference in the migration speed of SMCs according to the grooves. All the cytoskeletal fibers were reorganized in the same direction with grooves. Especially the alignments of microtubule and intermediate filaments were distinguished in the SMCs on the micro grooves.
CONCLUSION
These results could be applied to the analysis of vascular restenosis and the development of artificial blood vessels.
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