Prognostic Significance of Human Papillomavirus and Flow Cytometric Analysis of DNA Content in Cervical Cancer

Lee, Y H; Lee, K W; Kim, J O; Min, Y S; Kim, Y M; Lee, S H; Ku, P S

Abstract

Cancer of the uterine cervix is the most prevalent cancer in Korean women. Although the etiology of the cervical cancer has not been elucidated, it has been suggested that HPV infection is closely associated with the cancer. The prevalence of HPV in cervical cancer was 15~100%, and is supposed that introduction of HPV DNA into the cell is one of the mechanism in eliciting cervical cancer, And non-diploidy DNA is usually detected in HPV positive cancer, so introduction of HPV DNA into the cell may increase chromosomal instabiligy. In this study, to investigate the role of human papilomavirus(HPV) infection on DNA contednt of cervical cancer, 58 cases of cervicl cancer were studied with the method of flow cytometric analysis of DNA content and PCR for typing of HPV in combination with other clinical parameters. The results were as follows: 1. The detection rate of HPV type 16 was 62.3%(33/58). 2. The proportion of HPV type 16 positivity significantly increased with the clinical stage, whereas no correlation was found between HPV type 16 positivity and the other clinical and histological parameters. 3. In flow cytometric analysis, DNA non-diploidy was 67.3%(33.49), and no coorelation was found between DNA diploidy and clinical, histological parameters, but S phase fraction was significantly different between diploidy and non-diploidy group. 4. There was no correlatton between HPV type 16 and DNA diploidy. 5. The cumulative survival according to HPV type 16 positivity was not statistically significant. 6. The cumulative survival according to DNA diploidy and S phase fraction(15%) was not statisticall significant.