Effects of Substance P on Histamine and Leukotriene E4 Release from Rat Mast Cells according to Temperature Changes

Kim, Yong Bok; Park, Moon Suh; Lim, Hyun Joon; Cho, Joong Saeng; Cha, Chang Il

Abstract

It has been widely known that nasal mucosa shows reaction to neuromediators, to irritants, and to physical stimuli such as cold exposure. The mechanisms of hypersensitivity to cold were not fully understood. This study was performed to evaluate the relationship between thermal changes and releases of chemical mediators in mast cells. Mast cells were obtained from pleural and peritoneal fluids of 12 Sprague Dawley rats. They were stimulated with substance P and compound 48/80, known most powerful stimulant to mast cells, for 5 and 30 minutes under the temperature of 0degreesC, 10degreesC, 20degreesC, 25degreesC and 36degreesC. In control group, nontreated mast cells were incubated for 5 and 30 minutes under the same temperatures as experimental group. Histamine and leukotriene E4 were measured in supernatant of each culture tube by enzyme linked immunosorbent assay. Mast cells treated with compound 48/80 produced a marked increase in histamine release and the cells treated with substance P did an increased histamine too. The increases in substance P treated mast cells were less than those in compound 48/80 treated cells. However, there were statistically no difference of histamine release between substance P treated cells and compound 48/80 treated cells in 20degreesC. Contents of leukotriene E4 were too small to measure amounts in both control and compound 48/80 treated group. The cells treated with substance P showed a visible increase in leukotriene E4 release on 5 minutes incubation at 36degreesC and on 30 minutes incubation at 20degreesC. This study suggests that a thermal change has not an effect on releasing chemical mediators from mast cells and substance P could play an important role in release of chemical mediators at the environment of 20degreesC.