Expression of Intercellular Adhesion Molecule-1 after Ischemia Reperfusion Injury of the Canine Lung

Sung, Sook Whan; Kim, Young Tae; Kim, Mun Soo; Lee, Jae Ik; Kang, Moon Chul; Seo, Jeong Wook

Abstract

BACKGROUND: Predicting the important role of intercellular adhesion molecule-1 expression on the acute ischemia-reperfusion injury, we set out to demonstrate it by assessing the degree of expression of ICAM-1 after warm ischemia-reperfusion in canine unilateral lung ischemia model. MATERIAL AND METHOD: Left unilateral lung ischemia was induced by clamping the left hilum for 100 minutes in seven adult mongrel dogs. After reperfusion, various hemodynamic parameters and blood gases were analyzed for 4 hours, while intermittently clamping the right hilum in order to allow observation of the injured left lung function. The pulmonary venous blood was collected serially to measure TNF-alpha and cICAM-1 level. After 4 hours of reperfusion, the lung tissue was biopsied to assess ICAM-1 expression, and to measure tissue malondialdehyde(MDA) and ATP level. RESULT: The parameters including arterial oxygen partial pressure, pulmonary vascular resistance and tissue MDA and ATP level suggested severe lung damage. Serum TNF-alpha level was 8.76+/-2.37 ng/ml at 60 minutes after reperfusion and decreased thereafter. The cICAM-1 level showed no change after the reperfusion during the experiment. The tissue ICAM-1 expression was confirmed in 5 dogs.
CONCLUSION
The increase of TNF-alpha level and expression of tissue ICAM-1 were demonstrated after ischemia reperfusion injury in canine lung model. However, the difference in expression time of each component suggested that the blockage against ICAM-1 should be performed within 4 hours, and the blockage of TNF-alpha should be done within 60 minutes to achieve prevention of acute ischemia-reperfusion injury.