J Korean Diabetes Assoc.  2000 Aug;24(4):413-420.

Regulation of mFABP (fatty acid binding protein) Expression by PPAR in Cultured Human Skeletal Muscle Cell

Affiliations
  • 1Department of Internal Medicine, Seoul National University Medical Research Center, Seoul, Korea.
  • 2The Institute of Endocrinology, Seoul National University Medical Research Center, Seoul, Korea.
  • 3Nutrition and Metabolism research Center, Seoul National University Medical Research Center, Seoul, Korea.

Abstract

BACKGROUND: Fatty acid binding protein (FABP), putative mammalian fatty acid transporter, plays a role in fatty acid transport, the modulation of cellular signal transduction pathways and the protection against detergent like effects of fatty acids. FABP found in liver, adipose tissue, heart, skeletal muscle and FABP in skeletal muscle accounts for 2% of total protein mass. FABP expression has shown to be up-regulated by PPAR in liver and adipocyte. Adipocyte and liver FABP genes have a functional PPRE (PPAR responsive element) in their promoter region. This evidence led us to investigate for a possible the regulation of mFABP expression by PPAR in cultured human skeletal muscle cell.
METHODS
Myoblast were cultured in SkGM for 4weeks and were differentiated into myocyte in MEM for 4days. The myocytes were treated with PPAR ligand (troglitazone: 5 g/mL) or transduction with adenovirus-PPAR 1 (Ad-PPAR 1). mFABP expression was identified by northern blot.
RESULTS
mFABP expression was up-regulated by 4.0+/-1.2 fold in the PPAR ligand (p<0.05). There was increased in mFABP expression with transduction with adenovirus-PPAR 1 while there was no change in mFABP expression which transducted with adenovirus - -galactosidase.
CONCLUSION
These results demonstrates that mFABP expression is up-regulated by both PPAR ligand and by PPAR 1 over expression in cultured human skeletal muscle cells.

Keyword

Muscle fatty acid binding protein; PPAR 1; human skeletal muscle cell

MeSH Terms

Adenoviridae
Adipocytes
Adipose Tissue
Blotting, Northern
Carrier Proteins
Detergents
Fatty Acids
Heart
Humans*
Liver
Muscle Cells
Muscle, Skeletal*
Myoblasts
Peroxisome Proliferator-Activated Receptors*
Promoter Regions, Genetic
Signal Transduction
Carrier Proteins
Detergents
Fatty Acids
Peroxisome Proliferator-Activated Receptors
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