Korean J Clin Microbiol.
2004 Sep;7(2):111-118.
Prevalence of CTX-M-type Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea, 2003
- Affiliations
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- 1Department of Pediatrics, Kosin University College of Medicine, Korea.
- 2Department of Laboratory Medicine, Kosin University College of Medicine, Korea. kscpjsh@ns.kosinmed.or.kr
- 3Division of Food Microbiology, Korea Food and Drug Administration, Korea.
- 4Department of Laboratory Medicine, Keonyang University College of Medicine, Korea.
- 5Ajou University College of Medicine, Korea.
- 6Hanyang University College of Medicine, Korea.
- 7Sooncheonhyang University College of Medicine, Korea.
- 8Pochon CHA University College of Medicine, Korea.
- 9Chonnam National University Medical School, Korea.
- 10Yonsei University Wonju College of Medicine, Korea.
- 11The Catholic University of Korea College of Medicine, Korea.
- 12Seoul National University College of Medicine, Korea.
- 13Yonsei University College of Medicine, Korea.
Abstract
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BACKGROUND: The aims of this study were to survey nationwide susceptibilities of Escherichia coli and Klebsiella pneumoniae isolates against cefotaxime and to determine the prevalences of CTX-Mtype extended-spectrum beta-lactamases(ESBLs).
METHODS
During the period of February to July, 2003, E. coli and K. pneumoniae isolates were collected from 12 hospitals. Antimicrobial susceptibilities to cefotaxime were tested by the disk diffusion method. ESBL production was determined by the double disk synergy test. Cefotaxime-resistance of the ESBL-producers was transfered to E. coli DH5alphaand E. coli Top10-F by transformation. MICs of beta-lactam antibiotics were determined by the agar dilution method. Searches for blaCTX-M genes was performed by PCR amplication; pIs of beta-lactamases were determined by isoelectric focusing.
RESULTS
Among 230 isolates of E. coli and 232 isolates of K. pneumoniae, 27 (11.7%) and 79 (34.1%) were intermediate or resistant to cefotaxime, respectively. Twenty-four (10.4%) isolates of E. coli and 58 (25.0%) K. pneumoniae isolates showed positive results in the double disk synergy test. Three isolates of E. coli and 13 K. pneumoniae isolates harbored blaCTX-M-3 gene, 4 E. coli isolates harbored blaCTX-M-15 gene, and 1 E. coli and 5 K. pneumoniae isolates harbored blaCTX-M-14 gene.
CONCLUSION
E. coli and K. pneumoniae isolates producing CTX-M-type ESBLs were not uncommon in Korean hospitals. It is thought that periodical surveys are necessary for inspecting the spread of CTX-M-type ESBL genes are necessary.
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