J Lung Cancer.  2006 Jun;5(1):35-38. 10.6058/jlc.2006.5.1.35.

Mutational Analysis of Pro-apoptotic BAD Gene in Nonsmall Cell Lung Cancer

Affiliations
  • 1Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. suhulee@catholic.ac.kr

Abstract

PURPOSE : Evidence exists that deregulation of apoptosis is involved in the mechanisms of cancer development, and the somatic mutations of apoptosisrelated genes have been reported in human cancers. Bcl- XL/Bcl-2-associated death promoter (BAD), a pro-apoptotic member of Bcl-2 family, plays an important role in the intrinsic apoptosis pathway.
MATERIALS AND METHODS
: To explore the possibility that the genetic alterations of BAD might be involved in the development of human cancers, we analyzed the entire coding region and all splice sites of human BAD gene in 100 human non-small cell lung cancers (NSCLC) by polymerase chain reaction (PCR)-based single-strand conformation polymorphism (SSCP).
RESULTS
: The PCR-SSCP analysis detected no mutation in the entire coding regions and all splice sites of human BAD gene in the 100 NSCLCs.
CONCLUSION
: The data presented here suggested that BAD gene mutation may not contribute to the pathogenesis of human NSCLCs

Keyword

Non-small cell lung cancer; BAD; Apoptosis; Mutation

MeSH Terms

Apoptosis
Carcinoma, Non-Small-Cell Lung*
Clinical Coding
Humans
Lung Neoplasms
Polymerase Chain Reaction

Figure

  • Fig. 1. Representative SSCP of BAD gene in the non-small cell lung cancers. The exon 2 of the BAD gene were amplified by PGR using a specific primer set. The PCR products from the representative cases of non-small cell lung cancers were visualized on SSCP. SSCP of DNA from the non-small cell lung cancers (T) shows no aberrant bands as compared to SSCPs from the normal tissues (N),


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