Korean J Otolaryngol-Head Neck Surg.
1999 Jun;42(6):750-755.
The Effects of Retinoids on Expression of beta4 Integrin and Growth of the Head and Neck Squamous Cell Carcinoma Cell Lines
- Affiliations
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- 1Department of Otolaryngology, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, Korea. hkyoo@uuh.ulsan.kr
- 2Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Abstract
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BACKGROUND AND OBJECTIVES: Retinoids have been used in chemoprevention trials for a variety of epithelial malignancies. However, high incidence of toxicity and drug resistance remains as problems. Increase of the retinoid metabolism by cytochrome P450 has been known as one of the several mechanisms explaining these toxicity and the aquired resistance. There have been many studies about the biological effects of retinoids in head and neck squamous cell carcinoma (HNSCC), but there is no information about the effects of the retinoids metabolism on cell biology. The study presented here is designed to examine the relationship between cytochrome P450 induction and responses to retinoids in the HNSCC cell lines.
MATERIAL AND METHODS: We used a human HNSCC cell lines (AMC-HN-4, AMC-HN-6). The change of metabolic activity was analyzed using thin layer chromatography (TLC). The effects of retinoids on cell proliferation were evaluated with CellTiter 96 TMAQueous Cell Proliferation Assay. The effects of retinoids on beta4 integrin expression were evaluated with flow cytometry.
RESULTS
The inhibitory effects of retinoids on cell proliferation were higher in AMC-HN-4 cell line (cytochrome P450 uninducible) than in AMC-HN-6 cell line (cytochrome P450 inducible) (p<0.05). The expression of beta4 integrin was more effectively suppressed in the AMC-HN-4 cell line than in the AMC-HN-6 cell line.
CONCLUSION
The results of this study suggest that cytochrome P450 inducilbility may be an important factor to determine the biological effects of retinoids in HNSCC cells.
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