Ewha Med J.  2001 Mar;24(1):15-21. 10.12771/emj.2001.24.1.15.

Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model

Affiliations
  • 1Department of Otolaryngology, College of Medicine, Ewha Womans University, Korea.

Abstract


OBJECTIVE
To establish new in vitro model systems that better reflect in vivo condition, multicellular tumor spheroids(MTS) and raft culture were developed using cell lines of squamous cell carcinoma(SCCHN) of the head and neck in these 3-dimensional systems.
MATERIALS AND METHODS
Four SCCHN cell lines were used for MTS and raft culture.
RESULTS
All cell lines formed MTS, but only Tu-138 showed a good stratification at the airliquid interface in the raft culture system.
CONCLUSIONS
MTS and raft culture system were established successfully from the SCCHN cell lines.

Keyword

Multicellular tumor spheroid; Raft culture; Squamous cell carcinoma of the head and neck

MeSH Terms

Carcinoma, Squamous Cell*
Cell Line
Head*
Neck*
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